지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제10호
- 발행연도
- 2012.1
- 수록면
- 1,423 - 1,431 (9page)
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초록· 키워드
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The inhibitory effect of IgY against Vibrio parahaemolyticus and Vibrio vulnificus responsible for seafood-borne diseases was investigated in this study. Water-soluble fractions (WSF) of protein containing IgYs were isolated from the egg yolk of hens initially immunized with formalininactivated V. parahaemolyticus or V. vulnificus. Protein,total and specific IgY contents of the WSF were determined. The inhibitory and protective effects of IgYs on the growth of V. parahaemolyticus and V. vulnificus were assayed in liquid medium and in mice. IgYs showed high affinity to their corresponding antigens with high titer from day 28 onwards. Protein contents and total IgY concentrations remained stable throughout the immunization period, whereas specific IgY concentrations increased steadily and reached a plateau at day 49. Specific IgY powder (150 mg/ml) significantly inhibited further multiplication of both V. parahaemolyticus and V. vulnificus in liquid medium as compared with the control IgY. The bacteria count in mice feces was lower in mice pretreated with specific IgYs than in those pretreated with PBS or control IgY. Higher survival of mice was observed in the experimental groups pretreated with either anti-V.
parahaemolyticus (75% survival) or anti-V. vulnificus (87% survival) IgYs, compared with those in the control groups pretreated with PBS or nonspecific IgY. All mice in the control groups died within three days after bacteria inoculation; hence, the protective effect of specific IgYs against infection caused by V. parahaemolyticus and V.
vulnificus was demonstrated.
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참고문헌 (24)
참고문헌 신청
Akita, E. M. / 1992 / Immunoglobulins from egg yolk: Isolation and purification / J. Food Sci 57 : 629 ~ 634
Allen, J. C. / 1987 / Enzyme-linked immunoassay kits for routine food analysis / Trends Biotechnol 5 : 193 ~ 199
Austin, B. / 2010 / Vibrios as causal agents of zoonoses / Vet. Microbiol 140 : 310 ~ 317
Bauer, A. / 2006 / Occurrence of Vibrio parahaemolyticus, V. cholerae, and V. vulnificus in Norwegian Blue Mussels (Mytilus edulis) / Appl. Environ. Microbiol 72 : 3058 ~ 306
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Allen, J. C. / 1987 / Enzyme-linked immunoassay kits for routine food analysis / Trends Biotechnol 5 : 193 ~ 199
Austin, B. / 2010 / Vibrios as causal agents of zoonoses / Vet. Microbiol 140 : 310 ~ 317
Bauer, A. / 2006 / Occurrence of Vibrio parahaemolyticus, V. cholerae, and V. vulnificus in Norwegian Blue Mussels (Mytilus edulis) / Appl. Environ. Microbiol 72 : 3058 ~ 306
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
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