지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제1호
- 발행연도
- 2011.1
- 수록면
- 81 - 88 (8page)
이용수
초록· 키워드
오류제보하기
The anti-phytoplasma activities of surfactin (derived from Iranian native Bacillus subtilis isolates) and tetracycline towards Candidatus “Phytoplasma aurantifolia”, the agent of lime Witches’ broom disease, were investigated. HPLC was used to quantify the surfactin production in four previously characterized native surfactin-producing strains,and the one producing the highest amount of surfactin (about 1,500 mg/l) was selected and cultivated following optimized production and extraction protocols. Different combinations of purified surfactin and commercial tetracycline were injected into artificially phytoplasmainfected Mexican lime seedlings using a syringe injection system. An absolute quantitative real-time PCR system was developed to monitor the phytoplasma population shifts in the lime phloem during 3 months following the injections. The results revealed that the injections of surfactin or tetracycline had a significant inhibitory effect on Candidatus “P. aurantifolia”. However, the combined treatment with both surfactin and tetracycline (1:1)resulted in the highest inhibition due to a synergic effect,which suppressed the phytoplasma population from about 2×105 to less than 10 phytoplasma units/g plant tissue.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Abdel-Mawgoud, A. M / 2008 / Optimization of surfactin production by Bacillus subtilis isolate BS5 / Appl. Biochem. Biotechnol 150 : 305 ~ 325
Aldaghi, M / 2009 / A simple and rapid protocol of crude DNA extraction from apple trees for PCR and real-time PCR detection of ‘Candidatus Phytoplasma mali’ / J. Virol. Me
Alhudaib, K / 2009 / Molecular identification, potential vectors and alternative hosts of the phytoplasma associated with a lime decline disease in Saudi Arabia / Crop Prot 2
Arocha, Y / 2009 / Detection of phytoplasma and potyvirus pathogens in papaya (Carica papaya L) affected with ‘Bunchy Top Symptom’ (BTS) in eastern Cuba / Crop Prot 28 : 64
Bertaccini, A / 2007 / Phytoplasmas. Diversity, taxonomy, and epidemiology / Front. Biosci 12 : 673 ~ 689
Aldaghi, M / 2009 / A simple and rapid protocol of crude DNA extraction from apple trees for PCR and real-time PCR detection of ‘Candidatus Phytoplasma mali’ / J. Virol. Me
Alhudaib, K / 2009 / Molecular identification, potential vectors and alternative hosts of the phytoplasma associated with a lime decline disease in Saudi Arabia / Crop Prot 2
Arocha, Y / 2009 / Detection of phytoplasma and potyvirus pathogens in papaya (Carica papaya L) affected with ‘Bunchy Top Symptom’ (BTS) in eastern Cuba / Crop Prot 28 : 64
Bertaccini, A / 2007 / Phytoplasmas. Diversity, taxonomy, and epidemiology / Front. Biosci 12 : 673 ~ 689
이 논문의 저자 정보
최근 본 자료
댓글(0)
0