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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제9호
- 발행연도
- 2010.1
- 수록면
- 1,300 - 1,306 (7page)
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초록· 키워드
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Ethyl (R, S)-4-chloro-3-hydroxybutanoate (ECHB) is a useful chiral building block for the synthesis of L-carnitine and hypercholesterolemia drugs. The yeast reductase,YOL151W (GenBank locus tag), exhibits an enantioselective reduction activity, converting ethyl-4-chlorooxobutanoate (ECOB) exclusively into (R)-ECHB. YOL151W was generated in Escherichia coli cells and purified via Ni-NTA and desalting column chromatography. It evidenced an optimum temperature of 45oC and an optimum pH of 6.5-7.5. Bacillus subtilis glucose dehydrogenase (GDH)was also expressed in Escherichia coli, and was used for the recycling of NADPH, required for the reduction reaction. Thereafter, Escherichia coli cells co-expressing YOL151W and GDH were constructed. After permeablization treatment, the Escherichia coli whole cells were utilized for ECHB synthesis. Through the use of this system, the 30 mM ECOB substrate could be converted to (R)-ECHB.
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참고문헌 (21)
참고문헌 신청
Canovas, M / 2005 / Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into L-carnitine / Enzyme Microb. Technol 25 : 300 ~ 30
Chang, D / 2004 / Chemical and enzymatic synthetic methods for asymmetric oxidation of the C-C double bond / Biocatal. Biotransform 22 : 113 ~ 131
Choi, Y. H / 2010 / Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity / Appl. Microbiol. Biotechn
De Smet, M. J / 1978 / The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli / Biochim. Biophys. Acta 506 : 64 ~
Denyer, S. P / 2002 / Cellular impermeability and uptake of biocides and antibiotics in Gram-negative bacteria / J. Appl. Microbiol. Symp. Suppl 92 : 35S ~ 45S
Chang, D / 2004 / Chemical and enzymatic synthetic methods for asymmetric oxidation of the C-C double bond / Biocatal. Biotransform 22 : 113 ~ 131
Choi, Y. H / 2010 / Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity / Appl. Microbiol. Biotechn
De Smet, M. J / 1978 / The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli / Biochim. Biophys. Acta 506 : 64 ~
Denyer, S. P / 2002 / Cellular impermeability and uptake of biocides and antibiotics in Gram-negative bacteria / J. Appl. Microbiol. Symp. Suppl 92 : 35S ~ 45S
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