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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제7호
- 발행연도
- 2010.1
- 수록면
- 1,061 - 1,068 (8page)
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초록· 키워드
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The biological synthesis of nanoparticles has gained considerable attention in view of their excellent biocompatibility and low toxicity. We isolated and purified rhamnolipids from Pseudomonas aeruginosa strain BS-161R, and these purified rhamnolipids were used to synthesize silver nanoparticles. The purified rhamnolipids were further characterized and the structure was elucidated based on one- and two-dimensional 1H and 13C NMR, FT-IR, and HR-MS spectral data. Purified rhamnolipids in a pseudoternary system of n-heptane and water system along with n-butanol as a cosurfactant were added to the aqueous solutions of silver nitrate and sodium borohydride to form reverse micelles. When these micelles were mixed,they resulted in the rapid formation of silver nanoparticles.
The synthesized nanoparticles were characterized by UVVisible spectroscopy, transmission electron microscopy,and energy dispersive X-ray spectroscopy (EDS). The nanoparticles formed had a sharp adsorption peak at 410 nm, which is characteristic of surface plasmon resonance of the silver nanoparticles. The nanoparticles were monodispersed, with an average particle size of 15.1 nm (σ=±5.82 nm), and spherical in shape. The EDS analysis revealed the presence of elemental silver signal in the synthesized nanoparticles. The formed silver nanoparticles exhibited good antibiotic activity against both Grampositive and Gram-negative pathogens and Candida albicans,suggesting their broad-spectrum antimicrobial activity.
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참고문헌 (25)
참고문헌 신청
Abalos, A / 2001 / Physicochemical and antimicrobial properties of new rhamnolipids by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes / Langmuir 17 : 1367 ~ 137
Alt, V / 2004 / An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement / Biomaterials 25 : 4383 ~ 4391
Amsterdam, D / 1996 / Susceptibility testing of antimicrobials in liquid media in Antibiotics in Laboratory Medicine / Williams and Wilkins : 52 ~ 111
Baker, C / 2005 / Synthesis and antibacterial properties of silver nanoparticles / J. Nanosci. Nanotechnol 5 : 244 ~ 249
Baker, R. A / 1998 / Novel anthraquinones from stationary cultures of Fusarium oxysporum / J. Ferment. Bioeng 85 : 359 ~ 361
Alt, V / 2004 / An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement / Biomaterials 25 : 4383 ~ 4391
Amsterdam, D / 1996 / Susceptibility testing of antimicrobials in liquid media in Antibiotics in Laboratory Medicine / Williams and Wilkins : 52 ~ 111
Baker, C / 2005 / Synthesis and antibacterial properties of silver nanoparticles / J. Nanosci. Nanotechnol 5 : 244 ~ 249
Baker, R. A / 1998 / Novel anthraquinones from stationary cultures of Fusarium oxysporum / J. Ferment. Bioeng 85 : 359 ~ 361
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