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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제5호
- 발행연도
- 2009.1
- 수록면
- 525 - 529 (5page)
이용수
초록· 키워드
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Cytolethal distending toxins (CDTs) represent an emerging
family of newly described bacterial products that are
produced by a number of pathogens. The genes encoding
these toxins have been identified as a cluster of three
adjacent genes, cdtA, cdtB, and cdtC, plus 5 cdt genetic
variants, designated as cdt-I, cdt-II, cdt-III, cdt-IV, and cdt-
V, have been identified to date. In this study, a general
multiplex PCR system designed to detect Escherichia coli
cdts was applied to investigate the presence of cdt genes
among isolates. As a result, among 366 E. coli strains,
2.7% were found to carry the cdtB gene. In addition, the
use of type-specific primers revealed the presence of cdt-I,
cdtIV, and cdt-V types of the cdt gene, yet no cdt-II or cdt-
III strains. The presence of other virulence genes (stx1,
stx2, eae, bfp, espA, espB, and espD) was also investigated
using a PCR assay. Among the 10 cdtB gene-positive
strains, 8 were identified as CDT-producing typical
enteropathogenic E. coli (EPEC) strains (eae+, bfp+), whereas
2 were identified as CDT-producing atypical EPEC strains
(eae+, bfp-). When comparing the cytotoxic activity of the
CDT-producing typical and atypical EPEC strains, the
CDT-producing atypical EPEC strains appeared to be less
toxic than the CDT-producing typical EPEC strains.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Aktan, I. / 2004 / Characterization of attaching-effacing Escherichia coli isolated from animals at slaughter in England and Wales / Vet. Microbiol 102 : 43 ~ 53
Albert, M. J. / 1996 / Controlled study of cytolethal distending toxin-producing Escherichia coli infections in Bangladeshi children / J. Clin. Microbiol 34 : 717 ~ 719
Baldini, M. M. / 1983 / Plasmid-mediated adhesion in enteropathogenic Escherichia coli / J. Pediatr. Gastroenterol. Nutr 2 : 534 ~ 538
Bielaszewska, M. / 2004 / Characterization of cytolethal distending toxin genes and expression in Shiga toxin-producing Escherichia coli strains of non-O157 serogroups / Infe
Blanco, J. / 1996 / Polymerase chain reaction for detection of Escherichia coli strains producing cytotoxic necrotizing factor type 1 and type 2 (CNF1 and CNF2) / J. Microbio
Albert, M. J. / 1996 / Controlled study of cytolethal distending toxin-producing Escherichia coli infections in Bangladeshi children / J. Clin. Microbiol 34 : 717 ~ 719
Baldini, M. M. / 1983 / Plasmid-mediated adhesion in enteropathogenic Escherichia coli / J. Pediatr. Gastroenterol. Nutr 2 : 534 ~ 538
Bielaszewska, M. / 2004 / Characterization of cytolethal distending toxin genes and expression in Shiga toxin-producing Escherichia coli strains of non-O157 serogroups / Infe
Blanco, J. / 1996 / Polymerase chain reaction for detection of Escherichia coli strains producing cytotoxic necrotizing factor type 1 and type 2 (CNF1 and CNF2) / J. Microbio
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