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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 678 - 682 (5page)
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초록· 키워드
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The promoter region of a carbon starvation geneisolated from Pseudomonas putida was cloned and analyzedfor its potential use for in situ bioremediation and bioprocessing.We constructed a recombinant plasmid pMKD101 by cloningthe 0.65 kb promoter region of the gene into the promoterproving vector, pMK301, which contains the lacZ for β-galactosidase activity as a reporter gene. pMKD101 wastransformed into the wild-type P. putida MK1, resulting in P.putida RPD101, and analyzed for β-galactosidase activityunder different culture conditions. When RPD101 was grownon the minimal medium plus 0.1% glucose as a sole carbonsource in batch cultures, β-galactosidase activity was foundto be 3.2-fold higher during the stationary phase than duringthe exponential phase. In chemostat cultures, β-galactosidaseactivity was found to be 3.1-fold higher at the minimalgrowth rate (dilution rate=0.05 h-1) than at the maximalgrowth rate (dilution rate=0.173 h-1). The results suggest thata carbon starvation promoter can be utilized to maximize theexpression of a desired gene under nutrient limitation.
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참고문헌 (15)
참고문헌 신청
A / 1998 / Implications of thexylQ gene of TOL plasmid pWW102 for the evolution ofaromatic catabolic pathways : 1387 ~ 144 1396
A / 1999 / Effect of organic solvents on the yield of solvent-tolerantPseudomonas putida S12 : 2631 ~ 2635
Herrero / 1990 / Transposonvectors containing non-antibiotic resistance selection markersfor cloning and stable chromosomal insertion of foreigngenes in Gram-negative bacteri
Hopkins / 1993 / Microcosm and in situ field studies of enhancedbiotransformation of trichloroethylene by phenol-usingmicroorganisms : 2277 ~ 2285
In Y / 1987 / Survival and dormancy of bacteria : 1 ~ 108
A / 1999 / Effect of organic solvents on the yield of solvent-tolerantPseudomonas putida S12 : 2631 ~ 2635
Herrero / 1990 / Transposonvectors containing non-antibiotic resistance selection markersfor cloning and stable chromosomal insertion of foreigngenes in Gram-negative bacteri
Hopkins / 1993 / Microcosm and in situ field studies of enhancedbiotransformation of trichloroethylene by phenol-usingmicroorganisms : 2277 ~ 2285
In Y / 1987 / Survival and dormancy of bacteria : 1 ~ 108
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Cloning and sequencing of a starvation-driven promoter region from Pseudomonas putida
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Transcriptional Induction of a Carbon Starvation Gene during Other Starvation and Stress Challenges in Pseudomonas putida MK1 : A Role of a Carbon Starvation Gene in General Stravation and Stress Responses
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Studies on Genes and Biochemical Events Induced by Carbon-Source Starvation in Plant Cells
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