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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 347 - 353 (7page)
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초록· 키워드
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β-Glucosidase from Aspergillus usamii KCTC 6954 was used to convert ginsenoside Rb1 to compound K, which has a high bio-functional activity. The enzymatic activities during culturing for 15 days were determined using ρ-nitrophenyl-β-glucopyranoside. The growth rate of the strain and the enzymatic activity were maximized after 6 days (IU; 175.93 μM ml-1 min-1). The activities were maximized at 60oC in pH 6.0. During culturing, Rb1 was converted to Rd after 9 d and then finally converted to compound K at 15 d. In the enzymatic reaction, Rb1 was converted to the ginsenoside Rd within 1 h of reaction time and compound K could be detected after 8 h. As a result, this study demonstrates that Rb1→Rd→F2→compound K is the main metabolic pathway catalyzed by β-glucosidase and that β-glucosidase is a feasible option for the development of specific bioconversion processes to obtain minor ginsenosides such as Rd and compound K.
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참고문헌 (24)
참고문헌 신청
Barbagallo RN / 2004 / Selection, characterization and comparison of β-glucosidase from mould and yeasts employable for enological applications / Enzyme Microb. Technol 35 :
Chang KH / 2009 / Optimization of the enzymatic production of 20(S)-ginsenoside Rg3 from white ginseng extract using response surface methodology / New Biotechnol 26 : 181 ~
Chang KH / 2012 / Purification and characterization of a ginsenoside Rb1-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239 / Int. J. Mol. Sci 13 : 12140 ~ 12152
Cui CH / 2013 / Characterization of the ginsenoside-transforming recombinant beta-glucosidase from Actinosynnema mirum and bioconversion of major ginsenosides into minor gins
Fuzzati N / 2004 / Analysis methods of ginsenosides / J. Chromatogr. B 812 : 119 ~ 133
Chang KH / 2009 / Optimization of the enzymatic production of 20(S)-ginsenoside Rg3 from white ginseng extract using response surface methodology / New Biotechnol 26 : 181 ~
Chang KH / 2012 / Purification and characterization of a ginsenoside Rb1-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239 / Int. J. Mol. Sci 13 : 12140 ~ 12152
Cui CH / 2013 / Characterization of the ginsenoside-transforming recombinant beta-glucosidase from Actinosynnema mirum and bioconversion of major ginsenosides into minor gins
Fuzzati N / 2004 / Analysis methods of ginsenosides / J. Chromatogr. B 812 : 119 ~ 133
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