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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제3호
- 발행연도
- 2010.1
- 수록면
- 532 - 541 (10page)
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초록· 키워드
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Peroxidase-like activity of Vitreoscilla hemoglobin (VHb)has been recently disclosed. To maximize such activity,two catalytically conserved residues (histidine and arginine)found in the distal pocket of peroxidases have successfully been introduced into that of the VHb. A 15-fold increase in catalytic constant (kcat) was obtained in P54R variant,which was presumably attributable to the lower rigidity and higher hydrophilicity of the distal cavity arising from substitution of proline to arginine. None of the modifications altered the affinity towards either H2O2 or ABTS substrate.
Spectroscopic studies revealed that VHb variants harboring the T29H mutation apparently demonstrated a spectral shift in both ferric and ferrous forms (406-408 to 411 nm,and 432 to 424-425 nm, respectively). All VHb proteins in the ferrous state had a λsoret peak at ~419 nm following the carbon monoxide (CO) binding. Expression of the P54R mutant mediated the downregulation of iron superoxide dismutase (FeSOD) as identified by two-dimensional gel electrophoresis (2-DE) and peptide mass fingerprinting (PMF). According to the high peroxidase activity of P54R,it could effectively eliminate autoxidation-derived H2O2,which is a cause of heme degradation and iron release.
This decreased the iron availability and consequently reduced the formation of the Fe 2+-ferric uptake regulator protein (Fe 2+-Fur), an inducer of FeSOD expression.
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참고문헌 (21)
참고문헌 신청
Adams, P. A / 1990 / The peroxidasic activity of the haem octapeptide microperoxidase-8(MP-8): The kinetic mechanism of the catalytic reduction of H2O2 by MP-8 using 2,2-azin
Allocatelli, C. T / 1993 / Structural and functional characterization of sperm whale myoglobin mutants: Role of arginine (E10) in ligand stabilization / Biochemistry 32 : 604
Andersson, C. I / 2003 / Enhanced ribosome and tRNA contents in Escherichia coli expressing a truncated Vitreoscilla hemoglobin mutant analyzed by flow field-flow fractionati
Arseguel, D / 2004 / Removal of phenol from coupling of talc and peroxidase: Application for depollution of waste water containing phenolic compounds / J. Chem. Technol. Biot
Ascenzi, P / 1989 / pH effects on the haem iron co-ordination state in the nitric oxide and deoxy derivatives of ferrous horseradish peroxidase and cytochrome c peroxidase /
Allocatelli, C. T / 1993 / Structural and functional characterization of sperm whale myoglobin mutants: Role of arginine (E10) in ligand stabilization / Biochemistry 32 : 604
Andersson, C. I / 2003 / Enhanced ribosome and tRNA contents in Escherichia coli expressing a truncated Vitreoscilla hemoglobin mutant analyzed by flow field-flow fractionati
Arseguel, D / 2004 / Removal of phenol from coupling of talc and peroxidase: Application for depollution of waste water containing phenolic compounds / J. Chem. Technol. Biot
Ascenzi, P / 1989 / pH effects on the haem iron co-ordination state in the nitric oxide and deoxy derivatives of ferrous horseradish peroxidase and cytochrome c peroxidase /
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