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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제9호
- 발행연도
- 2006.1
- 수록면
- 1,384 - 1,391 (8page)
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The signal transduction system is one of the mostprotein kinases are included in the cellular signal transductionsystem. Finding a protein kinase inhibitor is very valuable,as it can be used to study cel biology and applied topharmaceuticals. For the efficient and rapid screening ofprotein kinase inhibitors, two asay systems were combined;the nonradioactive protein kinase assay system that uses anFITC-labeled IRS-2 peptide and the cel-based paper discassay system that uses Streptomyces griseus as the indicatorstrain. Among 330 kinds of herb extracts tested, the extractof Dalbergia odorifera exhibited the strongest inhibitoryactivity in the two assay systems and was selected for furtherisolation. Based on solvent extraction and many steps ofchromatography, seven compounds were finally separatedto homogeneity and their structures determined by 1H and13C NMR spectroscopies. Four were to be flavonoids andidentified as butin (C15H12O5, Mw=272.07), 3'-hydroxymelanetin(C16H12O6, Mw=300.06), liquiritigenin (C15H12O4, Mw=256.07),and 2'-hydroxyformononetin (C16H12O5, Mw=284.07). 3'-Hydroxymelanetin inhibited the phosphorylation of the GSK3protein by Akt to 37% at a concentration of 10g/ml andshowed the strongest cytotoxicity (ED50<50 g/ml) against thehuman cancer cell line HCT116. Under the same conditions,liquiritigenin also inhibited the phosphorylation of GSK3 bywas lower than 100g/ml.
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참고문헌 (24)
참고문헌 신청
[학술지(정
Bae, M / 1998 / Enhanced expression of Fas ligand is associated with aburatubolactam C-induced apoptosis in human Jurkat T cells / Biochem. Biophys. Res. Commun. 246 : 276 ~
Bourret, R. B / 1991 / Signal transduction pathways involving protein phosphorylation in prokaryotes / Annu. Rev. Biochem. 60 : 401 ~ 441
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem. 7
Chan, S. C / 1998 / Three new flavonoids and antiallergic, antiinflammatory constituents from the heartwood of Dalbergia odorifera / Planta Med. 64 : 153 ~ 158
Bae, M / 1998 / Enhanced expression of Fas ligand is associated with aburatubolactam C-induced apoptosis in human Jurkat T cells / Biochem. Biophys. Res. Commun. 246 : 276 ~
Bourret, R. B / 1991 / Signal transduction pathways involving protein phosphorylation in prokaryotes / Annu. Rev. Biochem. 60 : 401 ~ 441
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem. 7
Chan, S. C / 1998 / Three new flavonoids and antiallergic, antiinflammatory constituents from the heartwood of Dalbergia odorifera / Planta Med. 64 : 153 ~ 158
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