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The ptsHI operon from Leuconostoc mesenteroidesssp. mesenteroides SY1 (L. mesenteroides SY1), a strainisolated from kimchiptsH open reading frame (ORF) was 273 bp in size, whichcan encode a protein of 90 amino acid residues with amolecular weight of 9,212 Da. The ptsI ORF was 1,719 bp insize, which was capable of encoding a protein of 572 aminoacids with a molecular mass of 62,549 Da. ptsH and ptsIgenes were transcribed as a single transcript of 2.0 kb in sizeregardles of carbon sources, supporting the operon structure.Although the deduced amino acid sequences of the HPr andEI were highly homologous with those of other Gram-3rd amino acid) was present in HPr from L. mesenteroidesSY1. Phosphorylation sites of HPr included the histidineresidue (16th) and serine residue (47th). Mutant HPrs, in whicheach phosphorylation site was mutated into alanine, wereobtained, and phosphorylation with HPr and mutated HPrsshowed that HPr was phosphorylated at the serine residue(47th) by HPr kinase/phosphorylase (HPr K/P).

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