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자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제6호
발행연도
2006.1
수록면
870 - 879 (10page)

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The present study describes the formation ofsucinic acid by a nonvirulent, highly osmotolerant Klebsiellapneumoniae strain, its profile of metabolites, and enzymes ofthe sucinate production pathway. The strain producedsuccinate along with other metabolites such as lactate, acetate,and ethanol under aerobic as well as anaerobic growthconditions. The yield of succinate was higher in the presenceof MgCO3 under N2 atmosphere as compared with that underCO2 atmosphere. Analysis of intracellular metabolites showedthe presence of a smaller PEP pool than that of pyruvate.Oxaloacetate, citrate, and α-ketoglutarate pols were considerablylarger than those of isocitrate and fumarate. In order to understandthe synthesis of succinate, the enzymes involved in end-productformation were studied. Levels of phosphoenolpyruvatecarboxykinase, fumarate reductase, pyruvate kinase, and acetatekinase were higher under anaerobic growth conditions. Based onthe profiles of the metabolites and enzymes, it was concludedthat the synthesis of succinate tok place via oxaloacetate, malate,and fumarate in the strain under anaerobic growth. The strainSAP showed potential for the bioconversion of fumarate tosucinate under N2 atmosphere in the presence of MgCO3. Atan initial fumarate concentration of 10 g/l, 7.1 g/l fumaratewas converted to 7 g/l succinate with a molar conversionefficiency of 97.3%. The conversion efficiency and succinate yieldwere increased in the presence of glucose. Cels grown onfumarate contained an 18-fold higher fumarate reductase activityas compared with the activity obtained when grown on glucose.

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