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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 45 - 50 (6page)
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The chitosanase gene (btbchito) of Bacillus thuringiensis BMB171 was cloned and heterologously expressed in the yeast Pichia pastoris. After purification, about 300 mg of recombinant chitosanase was obtained from the 1-1 culture medium with a specific activity of 240 units/mg. Results determined by the combined use of thin layer chromatography (TLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) showed that the chitooligosaccharides (COSs) obtained by chitosan (N-deacetylated by 70%, 80%, and 90%) hydrolysis by rBTBCHITO were comprised of oligomers, with degrees of polymerization (DP) mainly ranging from trimers to heptamers; high molecular weight chitopentaose, chitohexaose, and chitoheptaose were also produced. Hydrolysis products was also deduced using MS since the COSs (n) are complex oligosaccharides with various acetyl groups from one to two, so the non-acetyl COSs (GlcN)n and COSs with more acetyls (> 2) were not detected. The employment of this method in the production of high molecular weight COSs may be useful for various industrial and biological applications, and the activity of chitosanase has great significance in research and other applications.
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참고문헌 (33)
참고문헌 신청
Park JK / 2011 / Effects of the molecular weight and the degree of deacetylation of chitosan oligosaccharides on antitumor activity / Int. J. Mol. Sci. 12:266~277
JEON,YOU-JIN / 2002 / Antitumor Activity of Chitosan Oligosaccharides Produced in Ultrafiltration Membrane Reactor System / Journal of Microbiology and Biotechnology 12(3):503~507
Lee BC / 2009 / In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus / Int. J. Mol. Med. 24:327~333
Takano S / 2012 / Progress in antimicrobial activities of chitin, chitosan and its oligosaccharides: A systematic study needs for food applications / Food Sci. Technol. Int. 18:3~34
Liu HT / 2009 / Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells / Pharmacol. Res. 59:167~175
JEON,YOU-JIN / 2002 / Antitumor Activity of Chitosan Oligosaccharides Produced in Ultrafiltration Membrane Reactor System / Journal of Microbiology and Biotechnology 12(3):503~507
Lee BC / 2009 / In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus / Int. J. Mol. Med. 24:327~333
Takano S / 2012 / Progress in antimicrobial activities of chitin, chitosan and its oligosaccharides: A systematic study needs for food applications / Food Sci. Technol. Int. 18:3~34
Liu HT / 2009 / Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells / Pharmacol. Res. 59:167~175
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