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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 79 - 83 (5page)
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초록· 키워드
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The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.
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등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Animal, Plant and Fisheries Quarantine and Inspection Agency / 2013 / List of plant quarantine viruses in Korea in newly revised in 2013 / Res. Plant Dis 19 : 67 ~ 75
Caruso P / 2003 / A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water / J. Microbiol. Methods 55 : 257 ~ 272
Hall TA / 1999 / BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT / Nucleic Acids Symp. Ser 41 : 95 ~ 98
Lee S. / 2013 / A Study of Molecular Biological Detection Methods for Seed-transmitted Viruses in Quarantine / Ph. D / Dankook University
Nelson M / 1992 / Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes / Methods Enzymol 216 : 279 ~ 303
Caruso P / 2003 / A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water / J. Microbiol. Methods 55 : 257 ~ 272
Hall TA / 1999 / BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT / Nucleic Acids Symp. Ser 41 : 95 ~ 98
Lee S. / 2013 / A Study of Molecular Biological Detection Methods for Seed-transmitted Viruses in Quarantine / Ph. D / Dankook University
Nelson M / 1992 / Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes / Methods Enzymol 216 : 279 ~ 303
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