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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제2호
- 발행연도
- 2013.1
- 수록면
- 167 - 176 (10page)
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초록· 키워드
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A novel bioactive molecule produced by Bacillus thuringiensis subsp. kurstaki Bn1 (Bt-Bn1), isolated from a common pest of hazelnut, Balaninus nucum L. (Coleoptera: Curculionidae),was determined, purified, and characterized in this study.
The Bt-Bn1 strain was investigated for antibacterial activity with an agar spot assay and well diffusion assay against B. cereus, B. weinhenstephenensis, L. monocytogenes,P. savastanoi, P. syringae, P. lemoignei, and many other B. thuringiensis strains. The production of bioactive molecule was determined at the early logarithmic phase in the growth cycle of strain Bt-Bn1 and its production continued until the beginning of the stationary phase. The mode of action of this molecule displayed bacteriocidal or bacteriolytic effect depending on the concentration. The bioactive molecule was purified 78-fold from the bacteria supernatant with ammonium sulfate precipitation, dialysis,ultrafiltration, gel filtration chromatography, and HPLC,respectively. The molecular mass of this molecule was estimated via SDS-PAGE and confirmed by the ESI-TOFMS as 3,139 Da. The bioactive molecule was also determined to be a heat-stable, pH-stable (range 6-8), and proteinase K sensitive antibacterial peptide, similar to bacteriocins.
Based on all characteristics determined in this study, the purified bacteriocin was named as thuricin Bn1 because of the similarities to the previously identified thuricin-like bacteriocin produced by the various B. thuringiensis strains. Plasmid elution studies showed that gene responsible for the production of thuricin Bn1 is located on the chromosome of Bt-Bn1. Therefore, it is a novel bacteriocin and the first recorded one produced by an insect originated bacterium. It has potential usage for the control of many different pathogenic and spoilage bacteria in the food industry, agriculture, and various other areas.
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참고문헌 (24)
참고문헌 신청
Abriouel, H / 2011 / Diversity and applications of Bacillus bacteriocins / FEMS Microbiol. Rev 35 : 201 ~ 232
Ahern, M / 2003 / Isolation and characterisation of a novel bacteriocin produced by Bacillus thuringiensis strain B439 / FEMS Microbiol. Lett 220 : 127 ~ 131
Anderson, I / 2005 / Comparative genome analysis of Bacillus cereus group genomes with Bacillus subtilis / FEMS Microbiol. Lett 250 : 175 ~ 184
Barboza-Corona, J. E / 2007 / Bacteriocin-like inhibitor substances produced by Mexican strains of Bacillus thuringiensis / Arch. Microbiol 187 : 117 ~ 126
Barboza-Corona, J. E / 2009 / Activity of bacteriocins synthesized by Bacillus thuringiensis against Staphylococcus aureus isolates associated to bovine mastitis / Vet. Micro
Ahern, M / 2003 / Isolation and characterisation of a novel bacteriocin produced by Bacillus thuringiensis strain B439 / FEMS Microbiol. Lett 220 : 127 ~ 131
Anderson, I / 2005 / Comparative genome analysis of Bacillus cereus group genomes with Bacillus subtilis / FEMS Microbiol. Lett 250 : 175 ~ 184
Barboza-Corona, J. E / 2007 / Bacteriocin-like inhibitor substances produced by Mexican strains of Bacillus thuringiensis / Arch. Microbiol 187 : 117 ~ 126
Barboza-Corona, J. E / 2009 / Activity of bacteriocins synthesized by Bacillus thuringiensis against Staphylococcus aureus isolates associated to bovine mastitis / Vet. Micro
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