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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제2호
- 발행연도
- 2011.1
- 수록면
- 162 - 169 (8page)
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초록· 키워드
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A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).
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참고문헌 (25)
참고문헌 신청
Chassagnole, C / 2002 / Dynamic modeling of the central carbon metabolism of Escherichia coli / Biotechnol. Bioeng 79 : 53 ~ 73
Doi,Y / 1990 / Microbial Polyesters / VCH Publishers
Faijes, M / 2007 / Comparision of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum / Microb. Cell Fact 6 : 27 ~
Fromm, H. J / 1962 / Kinetics studies of yeast hexokinase / J. Biol. Chem 237 : 3027 ~ 3032
Heinrich, R / 1974 / A linear steady state treatment of enzymatic chains . General properties, control and effector strength / Eur. J. Biochem 42 : 89 ~ 95
Doi,Y / 1990 / Microbial Polyesters / VCH Publishers
Faijes, M / 2007 / Comparision of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum / Microb. Cell Fact 6 : 27 ~
Fromm, H. J / 1962 / Kinetics studies of yeast hexokinase / J. Biol. Chem 237 : 3027 ~ 3032
Heinrich, R / 1974 / A linear steady state treatment of enzymatic chains . General properties, control and effector strength / Eur. J. Biochem 42 : 89 ~ 95
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