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자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제1호
발행연도
2010.1
수록면
94 - 100 (7page)

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Zymomonas mobilis was immobilized in a modified graphite felt cathode with neutral red (NR-cathode) and Saccharomyces cerevisiae was cultivated on a platinum plate anode. An electrochemical redox reaction was induced by 3 volts of electric potential charged to the cathode and anode. The Z. mobilis produced 1.3-1.5M of ethanol in the cathode compartment, whereas the S. cerevisiae produced 1.7-1.9M in the anode compartment after 96 h. The ethanol produced by the Z. mobilis immobilized in the NR-cathode and S. cerevisiae cultivated on the platinum plate was 1.5-1.6 times higher than that produced under conventional conditions. The electrochemical oxidation potential inhibited Z. mobilis, but activated S. cerevisiae. The SDS-PAGE pattern of the total soluble proteins extracted from the Z. mobilis cultivated under the electrochemical oxidation conditions was gradually simplified in proportion to the potential intensity. Z. mobilis and S. cerevisiae were cultivated in the cathode and anode compartments,respectively, of an electrochemical redox combination system. The Z. mobilis culture cultivated in the cathode compartment for 24 h was continuously transferred to the S. cerevisiae culture in the anode compartment at a rate of 300 ml/day. Approx. 1.0-1.2M of ethanol was produced by the Z. mobilis in the cathode compartment within 24 h,and an additional 0.8-0.9M produced by the S. cerevisiae in the anode compartment within another 24 h. Thus,a total of 2.0-2.1M of ethanol was produced by the electrochemical redox combination of Z. mobilis and S. cerevisiae within 48 h.

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