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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제1호
- 발행연도
- 2010.1
- 수록면
- 127 - 131 (5page)
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In this study, Corynebacterium glutamicum and its derived mutants were used to demonstrate the relationship between proline, glutamate, and ornithine. The maximum ornithine production was shown in the culture medium (3,295.0 mg/l)when the cells were cultured with 20 mM proline, and was 15.5 times higher than in the presence of 1 mM proline.
However, glutamate, which is known as an intermediate in the process of converting proline to ornithine, did not have any positive effect on ornithine production. This suggests that the conversion of proline to ornithine through glutamate, is not possible in C. glutamicum. Comparative analysis between the wild-type strain, SJC 8043 (argF-,argR-), and SJC 8064 (argF-, argR-, and ocd-), showed that C. glutamicum could regulate ornithine production by ornithine cyclodeaminase (Ocd) under proline-supplemented conditions. Therefore, proline directly caused an increase in the endogenous level of ornithine by Ocd, which would be a primary metabolite in the ornithine biosynthesis pathway.
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참고문헌 (23)
참고문헌 신청
Alam, S / 2004 / Crystallization and X-ray diffraction analysis of ornithine cyclodeaminase from Pseudomonas putida / Acta Crystallogr. D Biol. Crystallogr 60 : 941 ~ 944
Amund, O. O / 1983 / Increased L-ornithine production by an arg mutant of Acinetobacter lwoffi / Eur. Appl. Microbiol. Biotechnol 17 : 252 ~ 253
Chinard, F. P / 1952 / Photometric estimation of proline and ornithine / J. Biol. Chem 199 : 91 ~ 95
Chinen, A / 2007 / Innovative metabolic pathway design for efficient Lglutamate production by suppressing CO2 emission / J. Biosci. Bioeng 103 : 262 ~ 269
Cunin, R / 1986 / Biosynthesis and metabolism of arginine in bacteria / Microbiol. Rev 50 : 314 ~ 352
Amund, O. O / 1983 / Increased L-ornithine production by an arg mutant of Acinetobacter lwoffi / Eur. Appl. Microbiol. Biotechnol 17 : 252 ~ 253
Chinard, F. P / 1952 / Photometric estimation of proline and ornithine / J. Biol. Chem 199 : 91 ~ 95
Chinen, A / 2007 / Innovative metabolic pathway design for efficient Lglutamate production by suppressing CO2 emission / J. Biosci. Bioeng 103 : 262 ~ 269
Cunin, R / 1986 / Biosynthesis and metabolism of arginine in bacteria / Microbiol. Rev 50 : 314 ~ 352
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