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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제11호
- 발행연도
- 2009.1
- 수록면
- 1,470 - 1,474 (5page)
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초록· 키워드
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This paper describes the development a of direct multiplex
reverse transcription-nested polymerase chain reaction
(PCR) method, devised for simultaneous detection and
typing of influenza viruses. This method combines the direct
reverse transcription reaction without RNA purification
with the enhancement of sensitivity and specificity of
nested PCR. The method successfully detected three
major human influenza viruses: influenza virus A subtype 1
(H1N1) and subtype 3 (H3N2), and influenza B virus (B).
The minimum number of virus particles (pfu/ml) necessary
for detection in spiked saliva samples was 200 (H1N1), 140
(H3N2), and 4.5 (B). The method’s sensitivity and simplicity
will be convenient for use in clinical laboratories for the
detection and subtyping of influenza and possibly other
RNA viruses.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Chang, Hee Kyoung / 2008 / Development of Multiplex RT-PCR Assays for Rapid Detection and Subtypingof Influenza Type A Viruses from Clinical Specimens / Journal of Microbiolo
Chi, X. S. / 2007 / Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses / J. Virol. Methods 139 : 90 ~ 92
Choi, K. / 1979 / An evaluation of influenza mortality surveillance, 1962-1979. I. Time series forecasts of expected pneumonia and influenza deaths / Am. J. Epidemiol 113 : 2
Choi, K. / 1979 / An evaluation of influenza mortality surveillance, 1962-1979. II. Percentage of pneumonia and influenza deaths as an indicator of influenza activity / Am. J
Collins, R. A. / 2003 / A NASBA method to detect high- and lowpathogenicity H5 avian influenza viruses / Avian Dis 47 : 1069 ~ 1074
Chi, X. S. / 2007 / Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses / J. Virol. Methods 139 : 90 ~ 92
Choi, K. / 1979 / An evaluation of influenza mortality surveillance, 1962-1979. I. Time series forecasts of expected pneumonia and influenza deaths / Am. J. Epidemiol 113 : 2
Choi, K. / 1979 / An evaluation of influenza mortality surveillance, 1962-1979. II. Percentage of pneumonia and influenza deaths as an indicator of influenza activity / Am. J
Collins, R. A. / 2003 / A NASBA method to detect high- and lowpathogenicity H5 avian influenza viruses / Avian Dis 47 : 1069 ~ 1074
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