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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제11호
- 발행연도
- 2008.1
- 수록면
- 1,773 - 1,784 (12page)
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Effects of various industrially important carbon sources
(glucose, sucrose, xylose, gluconate, and glycerol) on
shikimic acid (SA) biosynthesis in Escherichia coli were
investigated to gain new insight into the metabolic capability
for overproducing SA. At the outset, constraints-based
flux analysis using the genome-scale in silico model of E.
coli was conducted to quantify the theoretical maximum
SA yield. The corresponding flux distributions fueled by
different carbon sources under investigation were compared
with respect to theoretical yield and energy utilization,
thereby identifying the indispensable pathways for achieving
optimal SA production on each carbon source. Subsequently,
a shikimate-kinase-deficient E. coli mutant was developed
by blocking the aromatic amino acid pathway, and
the production of SA on various carbon sources was
experimentally examined during 5 l batch culture. As a
result, the highest production rate, 1.92 mmol SA/h, was
obtained when glucose was utilized as a carbon source,
whereas the efficient SA production from glycerol was
obtained with the highest yield, 0.21 mol SA formed per
mol carbon atom of carbon source consumed. The current
strain can be further improved to satisfy the theoretically
achievable SA production that was predicted by in silico
analysis.
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참고문헌 (23)
참고문헌 신청
Bare-Viveros, J. L. / 2004 / Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli. Biotechn
Bockmann, J. / 3132 / Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132 / Mol. Gen. Genet 235 : 22 ~ 3
Bradley, D. / 2005 / Star role for bacteria in controlling flu pandemic? Nat. Rev / Drug Discov 4 : 945 ~
Chandran, S. S. / 2003 / Phosphoenolpyruvate availability and the biosynthesis of shikimic acid / Biotechnol. Prog 19 : 808 ~ 814
Datsenko, K. A. / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl / Acad. Sci. USA 97 : 6640 ~ 6645
Bockmann, J. / 3132 / Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132 / Mol. Gen. Genet 235 : 22 ~ 3
Bradley, D. / 2005 / Star role for bacteria in controlling flu pandemic? Nat. Rev / Drug Discov 4 : 945 ~
Chandran, S. S. / 2003 / Phosphoenolpyruvate availability and the biosynthesis of shikimic acid / Biotechnol. Prog 19 : 808 ~ 814
Datsenko, K. A. / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl / Acad. Sci. USA 97 : 6640 ~ 6645
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