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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제9호
- 발행연도
- 2008.1
- 수록면
- 1,544 - 1,549 (6page)
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초록· 키워드
오류제보하기
MhMTS and MhMTH are trehalose (α-D-glucopyranosyl-
[1,1]-α-D-glucopyranose) biosynthesis genes of the
thermophilic microorganism Metallosphaera hakonensis,
and encode a maltooligosyltrehalose synthase (MhMTS)
and a maltooligosyltrehalose trehalohydrolase (MhMTH),
respectively. In this study, the two genes were fused inframe
in a recombinant DNA, and expressed in Escherichia
coli to produce a bifunctional fusion enzyme, MhMTSH.
Similar to the two-step reactions with MhMTS and MhMTH,
the fusion enzyme catalyzed the sequential reactions on
maltopentaose, maltotriosyltrehalose formation, and following
hydrolysis, producing trehalose and maltotriose. Optimum
conditions for the fusion enzyme-catalyzed trehalose
synthesis were around 70oC and pH 5.0-6.0. The MhMTSH
fusion enzyme exhibited a high degree of thermostability,
retaining 80% of the activity when pre-incubated at 70oC
for 48 h. The stability was gradually abolished by incubating
the fusion enzyme at above 80oC. The MhMTSH fusion
enzyme was active on various sizes of maltooligosaccharides,
extending its substrate specificity to soluble starch, the
most abundant natural source of trehalose production.
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참고문헌 (21)
참고문헌 신청
CHOI, JEONG JIN / 2006 / Cloning, Expression, and Characterization of a Hyperalkaline Phosphatasefrom the Thermophilic Bacterium Thermus sp. T351 / Journal of Microbiology an
Eastmond, P. J. / 2003 / metabolism: A regulatory role for trehalose-6-phosphate. Curr. Opin / Plant Biol 6 : 231 ~ 235
Elbein, A. / 1974 / The metabolism of α,α-trehalose. Adv. Carbohydr. Chem / Biochem 30 : 227 ~ 256
Eleutherio, E. C. A. / 1993 / Protective role of trehalose during heat stress in Saccharomyces cerevisiae / Cryobiology 30 : 591 ~ 596
Giaever, H. M. / 1988 / Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli / Bacteriol 170 : 2841 ~ 2849
Eastmond, P. J. / 2003 / metabolism: A regulatory role for trehalose-6-phosphate. Curr. Opin / Plant Biol 6 : 231 ~ 235
Elbein, A. / 1974 / The metabolism of α,α-trehalose. Adv. Carbohydr. Chem / Biochem 30 : 227 ~ 256
Eleutherio, E. C. A. / 1993 / Protective role of trehalose during heat stress in Saccharomyces cerevisiae / Cryobiology 30 : 591 ~ 596
Giaever, H. M. / 1988 / Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli / Bacteriol 170 : 2841 ~ 2849
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