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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제8호
- 발행연도
- 2007.1
- 수록면
- 1,254 - 1,261 (8page)
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초록· 키워드
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In the present study, we have constructed abioluminescent bioreporter for the assesment of nitrate/nitritebioavailability in wastewater. Specifically, an approximately500-bp DNA fragment containing a nitrate/nitrite-activatednasR-like promoter (regulating expression of genes encodingnitrite reductase in the genus Klebsiella) was fused upstreamof the luxCDABE gene cassette in a modifiedmini-Tn5 vector. Characterization of this strain, designatedW6-1, yielded dose-dependent increased bioluminescencecoincident with increased nitrate, nitrite, and ammonium addedto the growth medium from 1 to 1 ppm. Bioluminescence inresponse to nitrogen species addition was light dependent upto 10, 7, and 8 ppm with nitrate, nitrite, and ammonium,respectively. This response was linear in the range from 1 to8 ppm for nitrate (R2=0.98), 1 to 6 ppm for nitrite (R2=0.99), and1 to 7 ppm for amonium (R2=0.9). A significant bioluminescentresponse was also recorded when strain W6-1 was incubatedwith sluries from aged, nitrate/nitrite contaminated wastewater.Thus, bioreporter strain W6-1 can be used to elucidate factorsthat constrain the use of nitrate/nitrite in wastewaters.
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참고문헌 (26)
참고문헌 신청
Abd-El-Haleem, D / 2002 / A luxCDABE-based bioluminescent bioreporter for the detection of phenol 29 : 233 ~ 237
Abd-El-Haleem, D / 2002 / Molecular characterization of phenol-degrading bacteria isolated from different Egyptian ecosystems 43 : 217 ~ 224
Abd-El-Haleem, D. / 2006 / Use of bioluminescent indicator Acinetobacter bacterium for screening and characterization of active antimicrobial agents 16 : 1706 ~ 1712
Addiscott, T. M. / 2004 / Nitrate and human health 20 : 98 ~ 104
Applegate, B. M / 1998 / A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing 64 : 2730 ~ 2735
Abd-El-Haleem, D / 2002 / Molecular characterization of phenol-degrading bacteria isolated from different Egyptian ecosystems 43 : 217 ~ 224
Abd-El-Haleem, D. / 2006 / Use of bioluminescent indicator Acinetobacter bacterium for screening and characterization of active antimicrobial agents 16 : 1706 ~ 1712
Addiscott, T. M. / 2004 / Nitrate and human health 20 : 98 ~ 104
Applegate, B. M / 1998 / A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing 64 : 2730 ~ 2735
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