지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제8호
- 발행연도
- 2007.1
- 수록면
- 1,242 - 1,248 (7page)
이용수
초록· 키워드
오류제보하기
Genomic analysis of a hyperthermophilic archaeon,Thermococcus onnurineus open reading frame consisting of 1,377 bp similar to α-amylasesfrom Thermococcales, encoding a 458-residue polypeptidecontaining a putative 25-residue signal peptide. The matureform of the α-amylase was cloned and the recombinant enzymewas characterized. The optimum activity of the enzyme occurredat 80oC and pH 5.5. The enzyme showed a liquefying activity,hydrolyzing maltoligosaccharides, amylopectin, and starchto producenot pullulan and cyclodextrin. Surprisingly, the enzyme wasnot highly thermostable, with half-life (t1/2) values of 10 min at90oC, despite the high similarity to α-amylases from Pyrococcus.Factors affecting the thermostability were considered to enhancethe thermostability. The presence of Ca2+ seemed to becritical, significantly changing t1/2 at 90oC to 153 min by theaddition of 0.5 mM Ca2+. On the other hand, the thermostabilitywas not enhanced by the addition of Zn2+ or other divalentmetals, irrespective of the concentration. The mutageneticstudy showed that the recovery of zinc-binding residues(His175 and Cys189) enhanced the thermostability, indicatingthat the residues involved in metal binding is very critical forthe thermostability.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
/ / A classification of glycosyl hydrolasesbased on amino acid sequence similarities : 309 ~ 316
A / / Pyrococcus furiosus α-amylase is stabilized by calcium andzinc : 6193 ~ 6201
Bae, S. S / 2006 / Thermococcus onnurineus sp. nov., a hyperthermophilicarchaeon isolated from a deep-sea hydrothermal vent area atthe PACMANUS field 16 : 1826 ~ 1831
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Choi, J. Y / 2006 / Expresionof thermostable α-glucosidase from Thermus caldophilusGK24 in recombinant Sacharomyces cerevisiae 16 : 2000 ~ 2003
A / / Pyrococcus furiosus α-amylase is stabilized by calcium andzinc : 6193 ~ 6201
Bae, S. S / 2006 / Thermococcus onnurineus sp. nov., a hyperthermophilicarchaeon isolated from a deep-sea hydrothermal vent area atthe PACMANUS field 16 : 1826 ~ 1831
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Choi, J. Y / 2006 / Expresionof thermostable α-glucosidase from Thermus caldophilusGK24 in recombinant Sacharomyces cerevisiae 16 : 2000 ~ 2003
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Hydrogen Production by the Hyperthermophilic Archaeon, Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2009 .05
-
Novel Na+-Dependent Respiration in Hyperthermophilic Archaeon, Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2014 .04
-
Adaptive Evolution of a Hyperthermophilic Archaeon Thermococcus onnurineus NA1 during Growth on Formate
한국미생물학회 학술대회논문집
2016 .04
-
Global Proteome Analysis of Sulfur-Reducing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2010 .05
-
Novel Deblocking Aminopeptidases of Hyperthermophilic Archaeon Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2010 .05
-
Immobilization of Hyperthermophilic Archaeon Thermococcus onnurineus NA1 on Amine-coated Silica Material for H₂ Production
한국미생물학회 학술대회논문집
2014 .04
-
Hydrogenogenic CO Metabolism of a Hyperthermophilic Archaeon Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2012 .05
-
Comparative Proteomic Analysis of Extracellular Vesicles (EVs) from the Hyperthermophilic Archaeon, Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2014 .04
-
Production of 2,3-Butanediol by Metabolically Engineered Hyperthermophilic Archaeon, Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2017 .04
-
Comparative Proteomics of Thermococcus onnurineus NA1 : Insights into the Sulfur Metabolism of a H₂-producing Hyperthermophilic Archaeon
한국미생물학회 학술대회논문집
2013 .05
-
A Novel CO-responsive Transcriptional Regulator and Its Manipulation to Enhance H2 Production in Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2015 .04
-
Hydrogen Production of Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2011 .05
-
Proteome Aanalyses of Hydrogen Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1 in Different One-carbon Substrate Culture Conditions
한국미생물학회 학술대회논문집
2012 .05
-
In-vitro Cloning, Expression, and Characterization of Carbon Monoxide Dehydrogenase (CODH) Catalytic Subunit from the Hyperthermophilic Archaeon Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2009 .05
-
Comparison of CO-dependent H₂ Production with Strong Promoters in Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2014 .04
-
A Novel Transcriptional Regulator of CO Metabolism In Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2013 .05
-
Proteome Analysis of Sulfur-Reducing Hyperthermophilic Archaea Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2009 .05
-
Genomic Analysis of a Marine Hyperthermophilic Archaeon Thermococcus sp. NAl
한국유전체학회 Conference
2005 .01
-
Hydrogenases and Hydrogen Production in Thermococcus onnurineus NA1
한국미생물학회 학술대회논문집
2009 .05
-
Immobilization of the Hyperthermophilic Archaeon Thermococcus onnurineus Using Amine-coated Silica Material for H2 Production
한국미생물·생명공학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0