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Paenibacillus polymyxa E681 is known to beable to suppress plant diseases by producing antimicrobialcompounds and to promote plant growth by producingphytohormones, and secreting diverse degrading enzymes. Inspite of these capabilities, little is known regarding theflow of information from the bacterial strain to the barleyroots. In an attempt to determine the flow of information fromthe presence and absence of barley, and two-dimensionalpolyacrylamide gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry were used. 2D-PAGE detectedapproximately 1,000 spots in the cell and 1,100 spots in thesupernatant at a pH 4-10 gradient. Interestingly, about 80spots from each sample showed quantitative variations. Fifty-thre spots from these were analyzed by MALDI-TOF massspectrometry and 28 proteins were identified. Most of thecytosolic proteins expressed at higher levels were found in P.polymyxain the absence of barley. Proteins detected at a lower level in thesurpernatant of P. polymyxa E681 cells grown in the presenceof barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase,heat-shock protein HtpG, spermidine synthase, OrfZ, ribonucleasePH, and coenzyme PQ synthesis protein, and flagelar hook-asociated protein 2 whereas proteins detected at a higher levelin the surpernatant of P. polymyxa E681 cells grown in the presenceof barley included D-alanyl-D-alanine ligase A, isopentenyl-diphosphate delta-isomerase, ABC transporter ATP-bindingprotein Uup, lipase. Many of the proteins belonging to plant-induced stimulons are associated with biosynthetic metabolismand metabolites of proteins and transport. Some of these proteinswould be expected to be induced by environmental changesresulting from the accumulation of plant-secreted substances.

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