지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 52 - 59 (8page)
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초록· 키워드
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The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett–Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, MnSO4 and FeSO4·6H2O were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, MgSO4 0.37 g/L, KH2PO4 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and NH4Cl (5:3 w/w) 10 g/L, KCl 0.16 g/L, FeSO4·6H2O 0.24 mg/L, MnSO4 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production (706.57 ± 3.70 mg/L) in the optimized medium confirmed the validity of the predicted model.
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참고문헌 (20)
참고문헌 신청
Amanullah, A. / 2001 / Scale - down model to simulate spatial pH variations in large - scale bioreactors / Biotechnol. Bioeng 73 : 390 ~ 399
Asaka, O. / 1996 / Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 / Appl. Environ. Microbiol 62 : 4081 ~ 4085
Bernheimer, A. / 1970 / Nature and properties of a cytolytic agent produced by Bacillus subtilis / J. Gen. Microbiol 61 : 361 ~
Chen, H. C. / 1996 / Optimizing the concentrations of carbon, nitrogen and phosphorus in a citric acid fermentation with response surface method / Food Biotechnol 10 : 13 ~ 2
Cooper, D. G. / 1991 / The response of Bacillus subtilis ATCC 21332 to manganese during continuous- phased growth / Appl. Microbiol. Biotechnol 35 : 72 ~ 76
Asaka, O. / 1996 / Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 / Appl. Environ. Microbiol 62 : 4081 ~ 4085
Bernheimer, A. / 1970 / Nature and properties of a cytolytic agent produced by Bacillus subtilis / J. Gen. Microbiol 61 : 361 ~
Chen, H. C. / 1996 / Optimizing the concentrations of carbon, nitrogen and phosphorus in a citric acid fermentation with response surface method / Food Biotechnol 10 : 13 ~ 2
Cooper, D. G. / 1991 / The response of Bacillus subtilis ATCC 21332 to manganese during continuous- phased growth / Appl. Microbiol. Biotechnol 35 : 72 ~ 76
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