지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 183 - 188 (6page)
이용수
초록· 키워드
오류제보하기
Seven isolates showing strong proteolytic activity, KYC 1028, 1100, 1134, 1139, 1151, 1159, and 1182, were collected. Out of them, the broth of KYC 1134 and KYC 1139 showed the high proteolytic activity measured
by azocazein. To determine 16S rDNA sequences for identification, 16S rDNA of seven isolates were amplified and compared with the 16S rDNA sequences of other myxobacteria at NCBI. It is evident from the phylogenetic
tree that the isolates belong to the genus Myxococcus. Sharing high percentage similarity values with myxobacteria, the 16S rDNA sequences were involved in two species, Myxococcus macrospores and M. Fulvus.
Biochemical characteristics of KYC 1134 broth, which showed the highest proteolytic activity, showed increased activity 8 times to seven days after culture, and protein production were increased gradually and
stopped at five days. The broth had optimal temperature at 60℃ for proteolytic activity, and stability of pH was ranged from pH 5 to 10, at 50℃ and 60, respectively. To classify proteases being in the broth, ten inhibitors
were determined and only bestatin showed 27% inhibition effect. The inhibition result demonstrates that the broth contains kinds of amino peptidases and other exopeptidases.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Bollag, D.M / 1995 / Epothilones, a new class of microtubule-stabilizing agents with Taxol-like mechanism of action / Cancer Res. 55 : 2325 ~ 2333
Bradford, M. M / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem
Gass. J / 2005 / Fermentation, Purification, Formulation, and Pharmacological Evaluation of a Prolyl Endopeptidase from Myxococcus Xanthus: Implication for Celiac Sprue Thera
Gerth, K / 1994 / The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (Myxobacteria). I. Soraphen A1 alpha: fermentation, isolation, biological pr
Gerth, K / 2003 / Myxobacteria: proficient producers of novel natural products with various biological activities-past and future / J. Biotechnol 106 : 233 ~ 253
Bradford, M. M / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem
Gass. J / 2005 / Fermentation, Purification, Formulation, and Pharmacological Evaluation of a Prolyl Endopeptidase from Myxococcus Xanthus: Implication for Celiac Sprue Thera
Gerth, K / 1994 / The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (Myxobacteria). I. Soraphen A1 alpha: fermentation, isolation, biological pr
Gerth, K / 2003 / Myxobacteria: proficient producers of novel natural products with various biological activities-past and future / J. Biotechnol 106 : 233 ~ 253
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