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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 70 - 75 (6page)
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초록· 키워드
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In Salmonella enterica, many genes encoded within Salmonella pathogenicity islands (SPI) 1 and 2 are required to cause a range of diseases in a variety of hosts. The SPI1-encoded regulator HilD activates both the SPI1 and 2 genes at different times during growth in Luria-Bertani (LB) media. In this study, the expression levels of hilD during growth in LB were investigated. The data suggest that hilD expression is induced in the early stationary phase and decreases in the late stationary phase, when sseA, an SPI2 gene, is maximally expressed. However, HilD could act as an activator of sseA expression in the late stationary phase despite being present at low levels. SseA expression was investigated in SPI1 regulator mutant strains, hilA, hilD and invF mutants. As expected, hilD mutation decreased sseA expression. However, we found that invF mutation caused a 1.5-fold increase in sseA expression in not only LB but also M9 minimal media, which is thought to resemble an intracellular environment. InvF overexpression restored sseA expression to wild-type levels in an invF mutant but did not cause an additional reduction in sseA expression. These results suggest that SPI1 controls SPI2 expression either positively or negatively.
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참고문헌 (18)
참고문헌 신청
Beuzon, C. R / 1999 / pH-dependent secretion of SseB, a product of the SPI-2 type III secretion system of Salmonella typhimurium / Mol. Microbiol 33 : 806 ~ 816
Boddicker, J. D / 2003 / Transcription of the Salmonella invasion gene activator, hilA, requires HilD activation in the absence of negative regulators / J. Bacteriol 185 : 52
Bustamante, V. H / 2008 / HilDmediated transcriptional cross-talk between SPI-1 and SPI-2 / Proc. Natl. Acad. Sci 105 : 14591 ~ 14596
Darwin, K. H. / 1999 / InvF is required for expression of genes encoding proteins secreted by the SPI1 type III secretion apparatus in Salmonella typhimurium / J. Bacteriol 1
Datsenko, K. A / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products / Proc. Natl. Acad. Sci 97 : 6640 ~ 6645
Boddicker, J. D / 2003 / Transcription of the Salmonella invasion gene activator, hilA, requires HilD activation in the absence of negative regulators / J. Bacteriol 185 : 52
Bustamante, V. H / 2008 / HilDmediated transcriptional cross-talk between SPI-1 and SPI-2 / Proc. Natl. Acad. Sci 105 : 14591 ~ 14596
Darwin, K. H. / 1999 / InvF is required for expression of genes encoding proteins secreted by the SPI1 type III secretion apparatus in Salmonella typhimurium / J. Bacteriol 1
Datsenko, K. A / 2000 / One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products / Proc. Natl. Acad. Sci 97 : 6640 ~ 6645
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