지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 1 - 9 (9page)
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초록· 키워드
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The genetic monitoring
method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in redpepper
field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in
the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC:1. 3. 1. 28]gene
(sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec -
1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural unsterilized soil was inoculated
with B. subtilis AH18 to determine the sensitivity (1.8×105 cfu/g) of multiplex PCR for the rapid dectection
and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper
cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere
soil.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Boldt, T. S / 2004 / Combined use of different gfp reporters for monitoring single-cell activities of a genetically modified PCB degrader in the rhizosphere of alfalfa / FEMS
Bourque, S. N / 1993 / Multiplex polymerase chain reaction for detection and differentiation of the microbial insecticide Bacillus thuringiensis / Appl. Environ. Microb 59 :
Cheun, H. I / 2003 / Erdenebaatar, K. Kawamoto, and I. Uchida. 2003. Rapid and effective detection of anthrax spores in soil by PCR / J. Appl. Microbiol 95 : 728 ~ 733
Demoz, B. T / 2006 / Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens / Biol. Control 37 : 68 ~ 74
Gera, R / 2003 / Monitoring rhizosphesre colonization by Azospirillum using constitutively expressed gfp gene / Ind. J. Microbiol 43 : 219 ~ 222
Bourque, S. N / 1993 / Multiplex polymerase chain reaction for detection and differentiation of the microbial insecticide Bacillus thuringiensis / Appl. Environ. Microb 59 :
Cheun, H. I / 2003 / Erdenebaatar, K. Kawamoto, and I. Uchida. 2003. Rapid and effective detection of anthrax spores in soil by PCR / J. Appl. Microbiol 95 : 728 ~ 733
Demoz, B. T / 2006 / Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens / Biol. Control 37 : 68 ~ 74
Gera, R / 2003 / Monitoring rhizosphesre colonization by Azospirillum using constitutively expressed gfp gene / Ind. J. Microbiol 43 : 219 ~ 222
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