지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 117 - 127 (11page)
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초록· 키워드
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The beneficial effects of adipose-derived stem cell conditioned media (ADSC-CM) for skin regeneration have previously been reported, despite the precise mechanism of how ADSC-CM promotes skin regeneration remaining unclear. ADSC-CM contains various secretomes and this may be a factor in it being a good resource for the treatment of skin conditions. It is also known that ADSC-CM produced in hypoxia conditions,in other words Advanced Adipose-Derived Stem cell Protein Extract (AAPE), has excellent skin regenerative properties. In this study, a human primary skin cell was devised to examine how AAPE affects human dermal fibroblast (HDF) and human keratinocyte (HK), which both play fundamental roles in skin regeneration. The promotion of collagen formation by HDFs was observed at 0.32 mg/ml of AAPE. AAPE treatment significantly stimulated stress fiber formation. DNA gene chips demonstrated that AAPE in HKs (p<0.05) affected the expression of 133 identifiable transcripts, which were associated with cell proliferation, migration, cell adhesion, and response to wounding. Twenty five identified proteins, including MMP, growth factor and cytokines such as CD54, FGF-2, GM-CSF, IL-4, IL-6, VEGF, TGF-β2, TGF-β3, MMP-1, MMP-10, and MMP-19, were contained in AAPE via antibody arrays. Thus, AAPE might activate the HK biological function and induce the collagen synthesis of HDF. These results demonstrate that AAPE has the potential to be used for clinic applications aimed at skin regeneration.
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참고문헌 (21)
참고문헌 신청
Adams, J. C / 1995 / Formation of stable microspikes containing actin and the 55 kDa actin bundling protein actin and the 55 kDa actin bundling protein, fascin, is a conseque
Bandyopadhyay, B / 2006 / A “traffic control” role for TGFbeta3: Orchestrating dermal and epidermal cell motility during wound healing / J. Cell Biol 172 : 1093 ~ 1105
Bevan, D. / 2004 / Diverse and potent activities of HGF/SF in skin wound repair / J. Pathol 203 : 831 ~ 838
Chen, B / 1999 / Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the sertoli cell-spermatid ectoplasmic specializat
Cramer, L. P / 1997 / Identification of novel graded polarity actin filament Bundles in locomoting heart fibroblasts: implications for the generation of motile force / J. Cel
Bandyopadhyay, B / 2006 / A “traffic control” role for TGFbeta3: Orchestrating dermal and epidermal cell motility during wound healing / J. Cell Biol 172 : 1093 ~ 1105
Bevan, D. / 2004 / Diverse and potent activities of HGF/SF in skin wound repair / J. Pathol 203 : 831 ~ 838
Chen, B / 1999 / Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the sertoli cell-spermatid ectoplasmic specializat
Cramer, L. P / 1997 / Identification of novel graded polarity actin filament Bundles in locomoting heart fibroblasts: implications for the generation of motile force / J. Cel
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