지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1 - 9 (9page)
이용수
초록· 키워드
오류제보하기
Histone deacetylases are involved in many biological processes and have roles in regulating cell behaviors such as cell cycle entry, cell proliferation and apoptosis. However, the effect of histone deacetylases on the development of hair cells (HCs) has not been fully elucidated. In this study, we examined the influence of histone deacetylases on the early development of neuromasts in the lateral line of zebrafish. Hair cell development was evaluated by fluorescent immunostaining in the absence or presence of histone deacetylase inhibitors. Our results suggested that pharmacological inhibition of histone deacetylases with inhibitors, including trichostatin A, valproic acid and MS-275, reduced the numbers of both HCs and supporting cells in neuromasts. We also found that the treatment of zebrafish larvae with inhibitors caused accumulation of histone acetylation and suppressed proliferation of neuromast cells. Real-time PCR results showed that the expression of both p21 and p27 mRNA was increased following trichostatin A treatment and the increase in p53 mRNA was modest under the same conditions. However, the expression of p53 mRNA was significantly increased by treatment with a high concentration of trichostatin A. A high concentration of trichostatin A also led to increased cell death in neuromasts as detected in a TUNEL assay. Moreover, the nuclei of most of these pyknotic cells were immunohistochemically positive for cleaved caspase-3. These results suggest that histone deacetylase activity is involved in lateral line development in the zebrafish and might have a role in neuromast formation by altering cell proliferation through the expression of cell cycle regulatory proteins.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Berger SL / 2002 / Histone modifications in transcriptional regulation / Curr Opin Genet Dev 12 : 142 ~ 148
Chuang DM / 2009 / Multiple roles of HDAC inhibition in neurodegenerative conditions / Trends Neurosci 32 : 591 ~ 601
Cole LK / 2001 / Apoptosis in the developing zebrafish embryo / Dev Biol 240 : 123 ~ 142
Cunliffe VT / 2004 / Histone deacetylase 1 is required to repress Notch target gene expression during zebrafish neurogenesis and to maintain the production of motoneurones i
Dalvai M / 2010 / The role of histone modifications and variants in regulating gene expression in breast cancer / J Mammary Gland Biol Neoplasia 15 : 19 ~ 33
Chuang DM / 2009 / Multiple roles of HDAC inhibition in neurodegenerative conditions / Trends Neurosci 32 : 591 ~ 601
Cole LK / 2001 / Apoptosis in the developing zebrafish embryo / Dev Biol 240 : 123 ~ 142
Cunliffe VT / 2004 / Histone deacetylase 1 is required to repress Notch target gene expression during zebrafish neurogenesis and to maintain the production of motoneurones i
Dalvai M / 2010 / The role of histone modifications and variants in regulating gene expression in breast cancer / J Mammary Gland Biol Neoplasia 15 : 19 ~ 33
이 논문의 저자 정보
최근 본 자료
댓글(0)
0