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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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- 학술저널
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- 2014.1
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The Cre/LoxP system is a well-established approach to spatially and temporally control genetic inactivation. The calcium/calmodulin-dependent protein kinase II alpha subunit (CaMKIIa) promoter limits expression to specific regions of the forebrainand thus has been utilized for the brain-specific inactivation of the genes. Here, we show that CaMKIIa-Cre can be utilized forsimultaneous inactivation of genes in the adult brain and in male germ cells. Double transgenic Rosa26þ/stop-lacZ::CaMKIIa-Creþ/Cre mice generated by crossing CaMKIIa-Creþ/Cre mice with floxed ROSA26 lacZ reporter (Rosa26þ/stop-lacZ) miceexhibited lacZ expression in the brain and testis. When these mice were mated to wild-type females, about 27% ofthe offspring were whole body blue by X-gal staining without inheriting the Cre transgene. These results indicate thatrecombination can occur in the germ cells of male Rosa26þ/stop-lacZ::CaMKIIa-Creþ/Cre mice. Similarly, when double transgenicGnaoþ/f::CaMKIIa-Creþ/Cre mice carrying a floxed Go-alpha gene (Gnaof/f) were backcrossed to wild-type females, approximately22% of the offspring carried the disrupted allele (GnaoD) without inheriting the Cre transgene. The GnaoD/D mice closelyresembled conventional Go-alpha knockout mice (Gnao / ) with respect to impairment of their behavior. Thus, we concludethat CaMKIIa-Cre mice afford recombination for both tissue- and time-controlled inactivation of floxed target genes in the brainand for their permanent disruption. This work also emphasizes that extra caution should be exercised in utilizing CaMKIIa-Cremice as breeding pairs.
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참고문헌 (27)
참고문헌 신청
Ackermann F / 2009 / CaMKIIalpha interacts with multi-PDZ domain protein MUPP1 in spermatozoa and prevents spontaneous acrosomal exocytosis / J Cell Sci 122 : 4547 ~ 4557
Branda CS / 2004 / Talking about a revolution: the impact of site-specific recombinases on genetic analyses in mice / Dev Cell 6 : 7 ~ 28
Burgin KE / 1990 / In situ hybridization histochemistry of Ca2þ/calmodulin-dependent protein kinase in developing rat brain / J Neurosci 10 : 1788 ~ 1798
Chamero P / 2011 / G protein G(alpha)o is essential for vomeronasal function and aggressive behavior in mice / Proc Natl Acad Sci USA 108 : 12898 ~ 12903
Cohen P / 2001 / Selective deletion of leptin receptor in neurons leads to obesity / J Clin Invest 108 : 1113 ~ 1121
Branda CS / 2004 / Talking about a revolution: the impact of site-specific recombinases on genetic analyses in mice / Dev Cell 6 : 7 ~ 28
Burgin KE / 1990 / In situ hybridization histochemistry of Ca2þ/calmodulin-dependent protein kinase in developing rat brain / J Neurosci 10 : 1788 ~ 1798
Chamero P / 2011 / G protein G(alpha)o is essential for vomeronasal function and aggressive behavior in mice / Proc Natl Acad Sci USA 108 : 12898 ~ 12903
Cohen P / 2001 / Selective deletion of leptin receptor in neurons leads to obesity / J Clin Invest 108 : 1113 ~ 1121
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