지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1 - 8 (8page)
이용수
초록· 키워드
오류제보하기
Type 1 diabetes is an autoimmune disease caused by permanent destruction of insulin-producing pancreatic b cells and requires lifelong exogenous insulin therapy. Recently, islet transplantation has been developed, and although there have been significant advances, this approach is not widely used clinically due to the poor survival rate of the engrafted islets. We hypothesized that improving survival of engrafted islets through ex vivo genetic engineering could be a novel strategy for successful islet transplantation. We transduced islets with adenoviruses expressing betacellulin, an epidermal growth factor receptor ligand, which promotes b-cell growth and differentiation, and transplanted these islets under the renal capsule of streptozotocin-induced diabetic mice. Transplantation with betacellulin-transduced islets resulted in prolonged normoglycemia and improved glucose tolerance compared with those of control virus-transduced islets. In addition, increased microvascular density was evident in the implanted islets, concomitant with increased endothelial von Willebrand factor immunoreactivity. Finally, cultured islets transduced with betacellulin displayed increased proliferation, reduced apoptosis and enhanced glucosestimulated insulin secretion in the presence of cytokines. These experiments suggest that transplantation with betacellulintransduced islets extends islet survival and preserves functional islet mass, leading to a therapeutic benefit in type 1 diabetes.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Chen S / 2007 / Reversal of streptozotocin-induced diabetes in rats by gene therapy with betacellulin and pancreatic duodenal homeobox-1 / Gene Ther 14 : 1102 ~ 1110
Jansson L / 2002 / Graft vascular function after transplantation of pancreatic islets / Diabetologia 45 : 749 ~ 763
Kawaguchi M / 2000 / Autoinduction and growth stimulatory effect of betacellulin in human pancreatic cancer cells / Int J Oncol 16 : 37 ~ 41
Kim HS / 2003 / Betacellulin induces angiogenesis through activation of mitogen-activated protein kinase and phosphatidylinositol 3’-kinase in endothelial cell / FASEB J 17
Kojima H / 2003 / NeuroD-betacellulin gene therapy induces islet neogenesis in the liver and reverses diabetes in mice / Nat Med 9 : 596 ~ 603
Jansson L / 2002 / Graft vascular function after transplantation of pancreatic islets / Diabetologia 45 : 749 ~ 763
Kawaguchi M / 2000 / Autoinduction and growth stimulatory effect of betacellulin in human pancreatic cancer cells / Int J Oncol 16 : 37 ~ 41
Kim HS / 2003 / Betacellulin induces angiogenesis through activation of mitogen-activated protein kinase and phosphatidylinositol 3’-kinase in endothelial cell / FASEB J 17
Kojima H / 2003 / NeuroD-betacellulin gene therapy induces islet neogenesis in the liver and reverses diabetes in mice / Nat Med 9 : 596 ~ 603
이 논문의 저자 정보
최근 본 자료
댓글(0)
0