지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 369 - 377 (9page)
이용수
초록· 키워드
오류제보하기
Microglial cells are the resident innate immune cells that sense pathogens and tissue injury in the central nervous system (CNS). Microglial activation is critical for neuroinflammatory responses. The synthetic compound 2-hydroxy-3',5,5'-trimethoxychalcone (DK-139)is a novel chalcone-derived compound. In this study,we investigated the effects of DK-139 on Toll-like receptor 4 (TLR4)-mediated inflammatory responses in BV2 microglial cells. DK-139 inhibited lipopolysaccharide (LPS)-induced TLR4 activity, as determined using a cell-based assay. DK-139 blocked LPS-induced phosphorylation of IκB and p65/RelA NF-κB, resulting in inhibition of the nuclear translocation and trans-acting activity of NF-κB in BV2 microglial cells. We also found that DK-139 reduced the expression of NF-κB target genes, such as those for COX-2, iNOS, and IL-1β, in LPS-stimulated BV2 microglial cells. Interestingly,DK-139 blocked LPS-induced Akt phosphorylation.
Inhibition of Akt abrogated LPS-induced phosphorylation of p65/RelA, while overexpression of dominant-active p110CAAX enhanced p65/RelA phosphorylation as well as iNOS and COX2 expression. These results suggest that DK-139 exerts an anti-inflammatory effect on microglial cells by inhibiting the Akt/IκB kinase (IKK)/NF-κB signaling pathway.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Barnes PJ / 1997 / Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases / N Engl J Med 336 : 1066 ~ 1071
Blasius AL / 2010 / Intracellular toll-like receptors / Immunity 32 : 305 ~ 315
Chow JC / 1999 / Toll-like receptor-4 mediates lipopolysaccharide- induced signal transduction / J Biol Chem 274 : 10689 ~ 10692
Czeh M / 2011 / The yin and yang of microglia / Dev Neurosci 33 : 199 ~ 209
Egawa K / 1999 / Membrane-targeted phosphatidylinositol 3-kinase mimics insulin actions and induces a state of cellular insulin resistance / J Biol Chem 274 : 14306 ~ 14314
Blasius AL / 2010 / Intracellular toll-like receptors / Immunity 32 : 305 ~ 315
Chow JC / 1999 / Toll-like receptor-4 mediates lipopolysaccharide- induced signal transduction / J Biol Chem 274 : 10689 ~ 10692
Czeh M / 2011 / The yin and yang of microglia / Dev Neurosci 33 : 199 ~ 209
Egawa K / 1999 / Membrane-targeted phosphatidylinositol 3-kinase mimics insulin actions and induces a state of cellular insulin resistance / J Biol Chem 274 : 14306 ~ 14314
이 논문의 저자 정보
최근 본 자료
댓글(0)
0