지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 293 - 302 (10page)
이용수
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Intrahepatic cholangiocarcinoma (ICC), a malignant tumor derived from the intrahepatic bile duct epithelium,has a poor prognosis and is refractory to conventional chemotherapy and radiation therapy. Thus,there is an urgent need to develop new effective therapeutic strategies for this disease. We previously found that L1 cell adhesion molecule (L1CAM) plays an important role in tumor progression of ICC, and we generated a murine mAb, A10-A3 (IgG1), that binds to the Ig1 domain of L1CAM. In the present study, we further characterized A10-A3, constructed a chimeric A10-A3antibody (cA10-A3) containing the constant regions of human IgG1, and evaluated the therapeutic potential in a human ICC xenograft nude mice model. The affinities (KD) of A10-A3 and cA10-A3 for soluble L1CAM were 1.8 nM and 1.9 nM, respectively, as determined by competition ELISA. A10-A3 inhibited L1CAM homophilic binding and was slowly internalized into the tumor cells, but it did not significantly inhibit proliferation of ICC cells in vitro. cA10-A3 mediated antibody-dependent cell-mediated cytotoxicity in vitro and displayed anti-tumor activity in the ICC animal model. These results suggest that the humanized A10-A3 antibody may have potential as an anticancer agent for the treatment of ICC.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Arlt MJ / 2006 / Efficient inhibition of intra-peritoneal tumor growth and dissemination of human ovarian carcinoma cells in nude mice by anti-L1-cell adhesion molecule monoc
Brummendorf T / 1995 / Cell adhesion molecules 1: immunoglobulin superfamily / Protein Profile 2 : 963 ~ 1108
Choi SY / 201110 / L1 cell adhesion molecule as a novel independent poor prognostic factor in gallbladder carcinoma / HUMAN PATHOLOGY 42 (10) : 1476 ~ 1483
Duczmal A / 1997 / The L1 adhesion molecule supports alpha v beta 3-mediated migration of human tumor cells and activated T lymphocytes / Biochem Biophys Res Commun 232 : 236
Friguet B / 1985 / Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay / J Immunol Methods 77 : 305 ~ 31
Brummendorf T / 1995 / Cell adhesion molecules 1: immunoglobulin superfamily / Protein Profile 2 : 963 ~ 1108
Choi SY / 201110 / L1 cell adhesion molecule as a novel independent poor prognostic factor in gallbladder carcinoma / HUMAN PATHOLOGY 42 (10) : 1476 ~ 1483
Duczmal A / 1997 / The L1 adhesion molecule supports alpha v beta 3-mediated migration of human tumor cells and activated T lymphocytes / Biochem Biophys Res Commun 232 : 236
Friguet B / 1985 / Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay / J Immunol Methods 77 : 305 ~ 31
이 논문의 저자 정보
최근 본 자료
댓글(0)
0