지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 446 - 454 (9page)
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Indoleamine 2,3-dioxygenase (IDO) is a key negative regulator of immune responses and has been implicated in tumor tolerance, autoimmune disease and asthma. IDO was detected in the joint synovial tissue in the inflammatory microenvironment of rheumatoid arthritis (RA), but IDO expression in joint synovial tissue is not sufficient to overcome the inflamed synovial environment. This study aimed to unravel the mechanisms involving the failure to activate tolerogenic IDO in the inflamed joint. We demonstrate that both poly (I:C) and lipopolysaccharide (LPS) induce expression of IDO in synovial fibroblasts. However, inflammatory cytokines such as IL-17, TNF-α, IL-12, IL-23 and IL-16did not induce IDO expression. Poly (I:C) appeared to induce higher IDO expression than did LPS. Surprisingly,toll-like receptor (TLR)4-mediated IDO expression was upregulated after depletion of myeloid differentiation primary response protein 88 (MyD88) in synovial fibroblasts using small interfering RNA (siRNA). IDO, TLR3 and TLR4 were highly expressed in synovial tissue of RA patients compared with that of osteoarthritis patients. In addition, RA patients with severe disease activity had higher levels of expression of IDO, TLR3 and TLR4 in the synovium than patients with mild disease activity. These data suggest that upregulation of IDO expression in synovial fibroblasts involves TLR3 and TLR4 activation by microbial constituents.
We showed that the mechanisms responsible for IDO regulation primarily involve MyD88 signaling in synovial fibroblasts, as demonstrated by siRNAmediated knockdown of MyD88.
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참고문헌 (23)
참고문헌 신청
Akira S / 2004 / Toll-like receptor signalling / Nat Rev Immunol 4 : 499 ~ 511
Beutelspacher SC / 2006 / Expression of indoleamine 2,3-dioxygenase (IDO) by endothelial cells: implications for the control of alloresponses / Am J Transplant 6 : 1320 ~ 133
Brentano F / 2005 / The role of Toll-like receptor signalling in the pathogenesis of arthritis / Cell Immunol 233 : 90 ~ 96
De Luca A / 2007 / Functional yet balanced reactivity to Candida albicans requires TRIF, MyD88, and IDOdependent inhibition of Rorc / J Immunol 179 : 5999 ~ 6008
Fujigaki S / 2001 / Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-gamma-independent mechanism / Eur J Immunol 31 : 2313 ~ 2318
Beutelspacher SC / 2006 / Expression of indoleamine 2,3-dioxygenase (IDO) by endothelial cells: implications for the control of alloresponses / Am J Transplant 6 : 1320 ~ 133
Brentano F / 2005 / The role of Toll-like receptor signalling in the pathogenesis of arthritis / Cell Immunol 233 : 90 ~ 96
De Luca A / 2007 / Functional yet balanced reactivity to Candida albicans requires TRIF, MyD88, and IDOdependent inhibition of Rorc / J Immunol 179 : 5999 ~ 6008
Fujigaki S / 2001 / Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-gamma-independent mechanism / Eur J Immunol 31 : 2313 ~ 2318
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