지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 167 - 175 (9page)
이용수
초록· 키워드
오류제보하기
The formation of advanced glycation end products (AGEs) has been considered to be a potential causative factor of injury to lens epithelial cells (LECs). Damage of LECs is believed to contribute to cataract formation.
The purpose of this study was to investigate the cytotoxic effect of AGEs on LECs both in vitro and in vivo.
We examined the accumulation of argpyrimidine, a methylglyoxal-derived AGE, and the expression of apoptosis-related molecules including nuclear factor-kappaB (NF-κB), Bax, and Bcl-2 in the human LEC line HLE-B3 and in cataractous lenses of Zucker diabetic fatty (ZDF) rats, an animal model of type 2diabetes. In cataractous lenses from twenty-oneweek-old ZDF rats, LEC apoptosis was markedly increased,and the accumulation of argpyrimidine as well as subsequent activation of NF-κB in LECs were significantly enhanced. The ratio of Bax to Bcl-2 protein levels was also increased. In addition, the accumulation of argpyrimidine triggered apoptosis in methylglyoxal-treated HLE-B3 cells. However, the presence of pyridoxamine (an AGEs inhibitor) and pyrrolidine dithiocarbamate (a NF-κB inhibitor) prevented apoptosis in HLE-B3 cells through the inhibition of argpyrimidine formation and the blockage of NF-κB nuclear translocalization, respectively. These results suggest that the cellular accumulation of argpyrimidine in LECs is NF-κB-dependent and pro-apoptotic.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Allen DA / 2005 / Mechanisms of high glucose-induced apoptosis and its relationship to diabetic complications / J Nutr Biochem 16 : 705 ~ 713
Ao S / 1991 / Effect of instillation of aldose reductase inhibitor FR74366 on diabetic cataract / Invest Ophthalmol Vis Sci 32 : 3078 ~ 3083
Baeuerle PA / 1994 / Function and activation of NF-kappa B in the immune system / Annu Rev Immunol 12 : 141 ~ 179
Bessho R / 1994 / Pyrrolidine dithiocarbamate, a potent inhibitor of nuclear factor kappa B (NF-kappa B) activation, prevents apoptosis in human promyelocytic leukemia HL-60
Boileau TW / 2003 / Ultraviolet radiation modulates nuclear factor kappa B activation in human lens epithelial cells / J Biochem Mol Toxicol 17 : 108 ~ 113
Ao S / 1991 / Effect of instillation of aldose reductase inhibitor FR74366 on diabetic cataract / Invest Ophthalmol Vis Sci 32 : 3078 ~ 3083
Baeuerle PA / 1994 / Function and activation of NF-kappa B in the immune system / Annu Rev Immunol 12 : 141 ~ 179
Bessho R / 1994 / Pyrrolidine dithiocarbamate, a potent inhibitor of nuclear factor kappa B (NF-kappa B) activation, prevents apoptosis in human promyelocytic leukemia HL-60
Boileau TW / 2003 / Ultraviolet radiation modulates nuclear factor kappa B activation in human lens epithelial cells / J Biochem Mol Toxicol 17 : 108 ~ 113
이 논문의 저자 정보
최근 본 자료
댓글(0)
0