지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 1 - 7 (7page)
이용수
초록· 키워드
오류제보하기
Glucagon-like peptide-1 (GLP-1) is a potent glucoincretin hormone and an important agent for the treatment of type 2 diabetes.
Here we demonstrate that B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic b-cells. GLP-1 treatment significantly increased BTG2, PDX-1 and insulin gene expression in pancreatic b-cells. Notably, adenovirus-mediated overexpression of BTG2 significantly elevated insulin secretion, as well as insulin and PDX-1 gene expression. Physical interaction studies showed that BTG2 is associated with increased PDX-1 occupancy on the insulin gene promoter via a direct interaction with PDX-1. Exendin-4 (Ex-4), a GLP-1 agonist, and GLP-1 in pancreatic b-cells increased insulin secretion through the BTG2–PDX-1–insulin pathway, which was blocked by endogenous BTG2 knockdown using a BTG2 small interfering RNA knockdown system. Finally, we revealed that Ex-4 and GLP-1 significantly elevated insulin secretion via upregulation of the BTG2–PDX-1 axis in pancreatic islets, and this phenomenon was abolished by endogenous BTG2 knockdown. Collectively, our current study provides a novel molecular mechanism by which GLP-1 positively regulates insulin gene expression via BTG2,suggesting that BTG2 has a key function in insulin secretion in pancreatic b-cells.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bradbury A / 1991 / Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization / Proc Natl Acad Sci USA 88 : 335
Chan G / 2011 / Molecular mechanism of pancreatic b-cell adaptive proliferation: studies during pregnancy in rats and in vitro / Endocrine 39 : 118 ~ 127
Deacon CF / 2004 / Therapeutic strategies based on glucagon-like peptide 1 / Diabetes 53 : 2181 ~ 2189
D’Alessio DA / 2004 / Glucagon-like peptide 1: evolution of an incretin into a treatment for diabetes / Am J Physiol Endocrinol Metab 286 : E882 ~ E890
Farioli-Vecchioli S / 2007 / Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3 / FASEB J 21 : 2215 ~ 2225
Chan G / 2011 / Molecular mechanism of pancreatic b-cell adaptive proliferation: studies during pregnancy in rats and in vitro / Endocrine 39 : 118 ~ 127
Deacon CF / 2004 / Therapeutic strategies based on glucagon-like peptide 1 / Diabetes 53 : 2181 ~ 2189
D’Alessio DA / 2004 / Glucagon-like peptide 1: evolution of an incretin into a treatment for diabetes / Am J Physiol Endocrinol Metab 286 : E882 ~ E890
Farioli-Vecchioli S / 2007 / Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3 / FASEB J 21 : 2215 ~ 2225
이 논문의 저자 정보
최근 본 자료
댓글(0)
0