지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 177 - 190 (14page)
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초록· 키워드
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Orai1 is the key subunit of the Ca2+-release-activated Ca2+ channel. Our previous report has demonstrated that Orai1 expression in the airway was upregulated in the ovalbumin (OVA)-induced allergic rhinitis (AR)mouse models. To observe whether inhibition of Orai1expression in the airway could suppress symptoms in a murine model of AR and to assess the impacts of this inhibition on the responses of local and systemic immunocytes,we administered recombinant lentivirus vectors that encoded shRNA against ORAI1 (lenti-ORAI1)into the nostrils of OVA-sensitized mice before the challenges, and analyzed its effect on allergic responses,as compared with the unsensitized mice and untreated AR mice. Administration of lenti-ORAI1 into the nasal cavity successfully infected cells in the epithelial layer of the nasal mucosa, and significantly decreased the frequencies of sneezing and nasal rubbing of the mice. Protein levels of leukotriene C4, OVA-specific IgE, and IL-4 in the nasal lavage fluid and serum and eosinophil cation protein in the serum were also significantly reduced by lenti-ORAI1, as were the mRNA levels of these factors in the nasal mucosa and spleen. These data suggested that administration of lenti-ORAI1 into the nasal cavity effectively decreased Orai1 expression in the nasal mucosa, alleviated AR symptoms, and partially inhibited the hyperresponsiveness of the local and systemic immune cells including T cells, B cells, mast cells and eosinophils that are involved in the pathogenesis of AR.
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참고문헌 (25)
참고문헌 신청
Allakhverdi Z / 2007 / Cutting edge: the ST2 ligand IL-33 potently activates and drives maturation of human mast cells / J Immunol 179 : 2051 ~ 2054
Baba Y / 2008 / Essential function for the calcium sensor STIM1 in mast cell activation and anaphylactic responses / Nat Immunol 9 : 81 ~ 88
Bandyopadhyay S / 2007 / Transcriptional regulation of T cell tolerance / Semin Immunol 19 : 180 ~ 187
Bhakta NR / 2005 / Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment / Nat Immunol 6 : 143 ~ 151
Borde M / 2006 / Transcriptional basis of lymphocyte tolerance / Immunol Rev 210 : 105 ~ 119
Baba Y / 2008 / Essential function for the calcium sensor STIM1 in mast cell activation and anaphylactic responses / Nat Immunol 9 : 81 ~ 88
Bandyopadhyay S / 2007 / Transcriptional regulation of T cell tolerance / Semin Immunol 19 : 180 ~ 187
Bhakta NR / 2005 / Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment / Nat Immunol 6 : 143 ~ 151
Borde M / 2006 / Transcriptional basis of lymphocyte tolerance / Immunol Rev 210 : 105 ~ 119
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