지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 26 - 35 (10page)
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초록· 키워드
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Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion).
hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes,osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled,3-dimensional islet cell-like clusters that express multiple genes related to pancreatic β-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.
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참고문헌 (25)
참고문헌 신청
Ae-Ri Ji / 2010 / Reactive oxygen species enhance differentiation of human embryonic stem cells into mesendodermal lineage / Experimental and Molecular Medicine 42 (3) : 175
Assady S / 2001 / Insulin production by human embryonic stem cells / Diabetes 50 : 1691 ~ 1697
Blyszczuk P / 2003 / Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells / Proc Natl Acad Sci USA 10
Bonner-Weir S / 2000 / In vitro cultivation of human islets from expanded ductal tissue / Proc Natl Acad Sci USA 97 : 7999 ~ 8004
Cha J / 2007 / Stem cells in cutaneous wound healing / Clin Dermatol 25 : 73 ~ 78
Assady S / 2001 / Insulin production by human embryonic stem cells / Diabetes 50 : 1691 ~ 1697
Blyszczuk P / 2003 / Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells / Proc Natl Acad Sci USA 10
Bonner-Weir S / 2000 / In vitro cultivation of human islets from expanded ductal tissue / Proc Natl Acad Sci USA 97 : 7999 ~ 8004
Cha J / 2007 / Stem cells in cutaneous wound healing / Clin Dermatol 25 : 73 ~ 78
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