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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 749 - 755 (7page)
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초록· 키워드
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Cinnamyl alcohol (CAL) is known as an antipyretic, and a recent study showed its vasodilatory activity without explaining the mechanism. Here we demonstrate the vasodilatory effect and the mechanism of action of CAL in rat thoracic aorta. The change of tension in aortic strips treated with CAL was measured in an organ bath system. In addition, vascular strips or human umbilical vein endothelial cells (HUVECs) were used for biochemical experiments such as Western blot and nitrite and cyclic guanosine monophosphate (cGMP)measurements. CAL attenuated the vasoconstriction of phenylephrine (PE, 1 μM)-precontracted aortic strips in an endothelium-dependent manner. CAL-induced vasorelaxation was inhibited by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10-4 M),methylene blue (MB; 10-5 M) and 1 H-[1,2,4]-oxadiazolole-[4,3-a] quinoxalin-10one, (ODQ; 10-6 or 10-7M) in the endothelium-intact aortic strips. Atrial natriuretic peptide (ANP; 10-8 or 10-9 M) did not affect the vasodilatory effect of CAL. The phosphorylation of endothelial nitric oxide synthase (eNOS) and generation of nitric oxide (NO) were stimulated by CAL treatment in HUVECs and inhibited by treatment with L-NAME. In addition,cGMP and PKG1 activation in aortic strips treated with CAL were also significantly inhibited by L-NAME. Furthermore, CAL relaxed Rho-kinase activator calpeptin-precontracted aortic strips, and the vasodilatory effect of CAL was inhibited by the ATP-sensitive K+ channel inhibitor glibenclamide (Gli; 10-5 M) and the voltage-dependent K+ channel inhibitor 4-aminopyridine (4-AP; 2 × 10-4 M). These results suggest that CAL induces vasorelaxation by activating K+ channels via the NO-cGMP-PKG pathway and the inhibition of Rho-kinase.
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참고문헌 (23)
참고문헌 신청
Browner NC / 2004 / Regulation of cGMP-dependent protein kinase expression by soluble guanylyl cyclase in vascular smooth muscle cells / J Biol Chem 279 : 46631 ~ 46636
Chai Y / 2008 / Dual regulation of the ATP-sensitive potassium channel by activation of cGMP-dependent protein kinase / Pflugers Arch 456 : 897 ~ 915
Chai Y / 2011 / Activation of cGMP-dependent protein kinase stimulates cardiac ATP-sensitive potassium channels via a ROS/calmodulin/CaMKII signaling cascade / PLoS One 6 : e
Chatterjee A / 2008 / Endothelial nitric oxide (NO) and its pathophysiologic regulation / Vascul Pharmacol 49 : 134 ~ 140
Chu DM / 1997 / Activation by cyclic GMP binding causes an apparent conformational change in cGMP-dependent protein kinase / J Biol Chem 272 : 31922 ~ 31928
Chai Y / 2008 / Dual regulation of the ATP-sensitive potassium channel by activation of cGMP-dependent protein kinase / Pflugers Arch 456 : 897 ~ 915
Chai Y / 2011 / Activation of cGMP-dependent protein kinase stimulates cardiac ATP-sensitive potassium channels via a ROS/calmodulin/CaMKII signaling cascade / PLoS One 6 : e
Chatterjee A / 2008 / Endothelial nitric oxide (NO) and its pathophysiologic regulation / Vascul Pharmacol 49 : 134 ~ 140
Chu DM / 1997 / Activation by cyclic GMP binding causes an apparent conformational change in cGMP-dependent protein kinase / J Biol Chem 272 : 31922 ~ 31928
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