지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 554 - 564 (11page)
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초록· 키워드
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Aptamers are synthetic, relatively short (e.g., 20-80 bases) RNA or ssDNA oligonucleotides that can bind targets with high affinity and specificity, similar to antibodies, because they can fold into unique, three-dimensional shapes. For use in various assays and experiments, aptamers have been conjugated with biotin or digoxigenin to form complexes with avidin or anti-digoxigenin antibodies, respectively. In this study, we developed a method to label the 5' ends of aptamers with cotinine, which allows formation of a stable complex with anti-cotinine antibodies for the purpose of providing another affinity unit for the application in biological assays using aptamers. To demonstrate the functionality of this affinity unit in biological assays,we utilized two well-known aptamers: AS1411, which binds nucleolin, and pegaptanib, which binds vascular endothelial growth factor. Cotinine-conjugated AS1411/ anti-cotinine antibody complexes were successfully applied to immunoblot, immunoprecipitation, and flow cytometric analyses, and cotinine-conjugated pegaptanib/ anti-cotinine antibody complexes were used successfully in enzyme immunoassays. Our results show that cotinine-conjugated aptamer/anti-cotinine antibody complexes are an effective alternative and complementary technique for aptamer use in multiple assays and experiments.
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참고문헌 (26)
참고문헌 신청
Backmann N / 2005 / A label-free immunosensor array using single-chain antibody fragments / Proc Natl Acad Sci USA 102 : 14587 ~ 14592
Baldrich E / 2005 / Displacement enzyme linked aptamer assay / Anal Chem 77 : 4774 ~ 4784
Bates PJ / 1999 / Antiproliferative activity of G-rich oligonucleotides correlates with protein binding / J Biol Chem 274 : 26369 ~ 26377
Bender DA / 1999 / Optimum nutrition: thiamin, biotin and pantothenate / Proc Nutr Soc 58 : 427 ~ 433
Blank M / 2001 / Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. selective targeting of endothelial regulatory protein pigpen / J Biol Chem 276
Baldrich E / 2005 / Displacement enzyme linked aptamer assay / Anal Chem 77 : 4774 ~ 4784
Bates PJ / 1999 / Antiproliferative activity of G-rich oligonucleotides correlates with protein binding / J Biol Chem 274 : 26369 ~ 26377
Bender DA / 1999 / Optimum nutrition: thiamin, biotin and pantothenate / Proc Nutr Soc 58 : 427 ~ 433
Blank M / 2001 / Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. selective targeting of endothelial regulatory protein pigpen / J Biol Chem 276
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