지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 494 - 500 (7page)
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초록· 키워드
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Paraquat (1,1’-dimethyl-4,4’-bipyridinium dichloride;PQ), an effective and widely used herbicide, was commercially introduced in 1962. It is reduced by the electron donor NADPH, and then reduced PQ transfers the electrons to molecular oxygen, resulting in the production of reactive oxygen species (ROS), which are related to cellular toxicity. However, the influence of continuous hypoxia on PQ-induced ROS production has not fully been investigated. We evaluated in vitro the protective effect of continuous hypoxia on PQinduced cytotoxicity in the human carcinogenic alveolar basal epithelial cell line (A549 cells) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and live and dead assay, and by measuring lactate dehydrogenase (LDH) release. To elucidate the mechanism underlying this effect, we monitored the immunofluorescence of intracellular ROS and measured malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx)activities. Continuous hypoxia protected the A549 cells from PQ-induced cytotoxicity. Continuous hypoxia for a period of 24 h significantly reduced intracellular ROS,decreased MDA concentration in the supernatant, and normalized SOD and GPx activities. Continuous hypoxia attenuated PQ-induced cell toxicity in A549cells. This protective effect might be attributable to the suppression of PQ-induced ROS generation.
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참고문헌 (27)
참고문헌 신청
Baker MA / 1990 / Microtiter plate assay for the measurement of glutathioneand glutathione disulfide in large numbers of biological samples / Anal Biochem 190 : 360 ~ 365
Bastianetto S / 2000 / The Ginkgo biloba extract (EGb 761) protects hippocampal neurons against cell death induced by beta-amyloid / Eur J Neurosci 12 : 1882 ~ 1890
Bus JS / 1974 / Superoxide- and singlet oxygencatalyzed lipid peroxidation as a possible mechanism for paraquat (methyl viologen) toxicity / Biochem Biophys Res Commun 58 : 7
Bus JS / 1984 / Paraquat: model for oxidant-initiated toxicity / Environ Health Perspect 55 : 37 ~ 46
Chang X / 2009 / Pyrrolidine dithiocarbamate attenuates paraquat-induced lung injury in rats / J Biomed Biotechnol 2009 : 619487 ~
Bastianetto S / 2000 / The Ginkgo biloba extract (EGb 761) protects hippocampal neurons against cell death induced by beta-amyloid / Eur J Neurosci 12 : 1882 ~ 1890
Bus JS / 1974 / Superoxide- and singlet oxygencatalyzed lipid peroxidation as a possible mechanism for paraquat (methyl viologen) toxicity / Biochem Biophys Res Commun 58 : 7
Bus JS / 1984 / Paraquat: model for oxidant-initiated toxicity / Environ Health Perspect 55 : 37 ~ 46
Chang X / 2009 / Pyrrolidine dithiocarbamate attenuates paraquat-induced lung injury in rats / J Biomed Biotechnol 2009 : 619487 ~
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