지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 25 - 30 (6page)
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To compare antioxidant and antibacterial effects of pine needles of pine trees (Pinus densiflora Seib. et Zucc.)that naturally grow in Gangwon-do, Republic of Korea, on human skin pathogens, extracts of pine needles were produced by two different extraction methods, using 80% ethanol and hydrothermal fluid methods. Then, they were used in the experiment after freeze drying both extracts and dissolving with 80% ethanol. Our analysis found that hydrothermal extraction method showed 4 times higher recovery rates compared with extraction with 80% ethanol. The reactive oxygen species (ROS) scavenging activity of these extracts by 1, 1-diphenyl-2-picryl hydrazyl assay was higher than L-ascorbic acid. In paper disc method examining antibacterial effects of these on human skin pathogens, such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus),Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans), and Propionibacterium acnes (P. acnes), 80% ethanol and hydrothermal extracts both displayed antibacterial effects against E. coli, S.
aureus, and P. acnes. Pine needle extracts presented especially high antibacterial effects against P. acnes.
However, Pine needle extracts showed insignificant antibacterial effect against P. aeruginosa and C. albicans.
In general, 80% ethanol extracts displayed greater antibacterial effects compared with hydrothermal extracts.
In conclusion, pine needle extracts prepared by both 80% ethanol and hydrothermal methods can be used as antioxidant production. In addition, our results showed that hydrothermal extraction was more suitable for extract production and ethanol extraction was more suitable for antibacterial effects.
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참고문헌 (24)
참고문헌 신청
Biois MS / 1958 / Antioxidant determination by the use of a stable free radical / Nature 26 : 1199 ~ 1200
Kang YH / 1995 / Studies on the physiological functionality of pine needle and mugwort extracts / Kor. J. Food Aci. Technol 27 : 978 ~
Kim KY / 2000 / Antimicrobial effectiveness of pine needle extract on foodborne illness bacteria / Kor. Soc. Microbiol. Biotechnol 10 : 227 ~ 232
Koukos PK / 2000 / Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce Grisebach) grown in Northern Greece / J. Agric. Food Chem 48 : 1
Packer L / 1999 / Antioxidant activity and biologic properties of a procyanidin rich extract from pine (Pinus maritima) bark, pycnogenol / Free Radic. Biol. Med 27 : 704 ~ 7
Kang YH / 1995 / Studies on the physiological functionality of pine needle and mugwort extracts / Kor. J. Food Aci. Technol 27 : 978 ~
Kim KY / 2000 / Antimicrobial effectiveness of pine needle extract on foodborne illness bacteria / Kor. Soc. Microbiol. Biotechnol 10 : 227 ~ 232
Koukos PK / 2000 / Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce Grisebach) grown in Northern Greece / J. Agric. Food Chem 48 : 1
Packer L / 1999 / Antioxidant activity and biologic properties of a procyanidin rich extract from pine (Pinus maritima) bark, pycnogenol / Free Radic. Biol. Med 27 : 704 ~ 7
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