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Ultraviolet B (UVB) radiation accelerates skin aging by inducing direct DNA damage and indirect intracellulardamage, which induce cell cycle arrest and apoptosis in human dermal fibroblasts (NHDFs). In this study,we demonstrated that pretreatment with the hexane extract of Chlorella vulgaris microalgae protects UVBmediatedcell damages in NHDFs. Firstly, we compared the cytotoxicity effect of four extracts (ethanol,hexane, ethyl acetate and distilled water) of Chlorella vulgaris on NHDFs, and found that the four extractshad a little cytotoxicity at 1-20 μg/ml. Additional biochemical assay showed that UVB-induced cytotoxicitywas significantly reduced in NHDFs posttreated with only hexane extract of Chlorella vulgaris . Furthermore,to increase the UVB-protection effect of the extract, we changed the concentration and treatment time, andfound that 48 h of posttreatment with hexane extract (20 μg/ml) of Chlorella vulgaris significantly increasedthe cell viability by 34.01±5.86% compared with the viability of UVB-irradiated NHDFs. In addition, we foundthat the hexane extract of Chlorella vulgaris promoted the DNA repair properties in NHDFs, as revealed byluciferase-based DNA repairing assay. Overall, our results indicate that hexane extract of Chlorella vulgarisis implicated as a potential chemopreventive agent through UVB protection effect on NHDFs.

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