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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Background: Cyclo-oxygenase-2 (Cox-2) is an inflammatory mediator that is necessaryfor the tissue repair, including bone fracture healing. Although the application of Cox-2gene therapy to a murine closed femoral fracture has accelerated bony union, but thebeneficial effect was not observed until the endochondral stage of bone repair that iswell after the inflammatory stage normally subsides. Methods: To identify the molecularpathways through which Cox-2 regulates fracture healing, we examined gene expres-sion profile in fracture tissues in response to Cox-2 gene therapy during the endochon-dral bone repair phase. Cox-2 gene therapy was applied to the closed murine femurfracture model. Microarray analysis was performed at 10 days post-fracture to examineglobal gene expression profile in the fracture tissues during the endochondral bone re-pair phase. The entire repertoire of significantly expressed genes was examined by geneset enrichment analysis, and the most up-regulated individual genes were evaluatedfurther. Results: The genes that normally promote inflammation were under-represent-ed in the microarray analysis, and the expression of several inflammatory chemokineswas significantly down-regulated. There was an up-regulation of two key transcriptionfactor genes that regulate hematopoiesis and erythropoiesis. More surprisingly, therewas no significant up-regulation in the genes that are normally involved in angiogenesisor bone formation. However, the expression of two tissue remodeling genes was up-regulated. Conclusions: The down-regulation of the inflammatory genes in response toCox-2 gene therapy was unexpected, given the pro-inflammatory role of prostaglandins.
Cox-2 gene therapy could promote bony union through hematopoietic precursor prolif-eration during endochondral bone repair and thereby enhances subsequently fracturecallus remo deling that leads to bony union of the fracture gap.
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참고문헌 (24)
참고문헌 신청
Amano H. / 2003 / Host prostaglandin E(2)-EP3 signaling regulates tumor-associated angiogenesis and tumor growth / J Exp Med. 197 : 221 ~ 232
Axelrad TW. / 2007 / New technologies for the enhancement of skeletal repair / Injury. 38 (Suppl 1) : S49 ~ 62
Bolander ME. / 1992 / Regulation of fracture repair by growth factors / Proc Soc Exp Biol Med. 200 : 165 ~ 170
Bonnarens F. / 1984 / Production of a standard closed fracture in laboratory animal bone / J Orthop Res. 2 : 97 ~ 101
Fujita H. / 2013 / Prevention of bone loss after ovariectomy in mice with preferential overexpression of the transcription factor paired box-5 in osteoblasts / Biol Pharm Bu
Axelrad TW. / 2007 / New technologies for the enhancement of skeletal repair / Injury. 38 (Suppl 1) : S49 ~ 62
Bolander ME. / 1992 / Regulation of fracture repair by growth factors / Proc Soc Exp Biol Med. 200 : 165 ~ 170
Bonnarens F. / 1984 / Production of a standard closed fracture in laboratory animal bone / J Orthop Res. 2 : 97 ~ 101
Fujita H. / 2013 / Prevention of bone loss after ovariectomy in mice with preferential overexpression of the transcription factor paired box-5 in osteoblasts / Biol Pharm Bu
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