갑상선질환에서 Sonic Hedgehog 단백질 발현의 임상적 의의

유근수; 이옥준; 김원재; 박성수; 김동주; 박진우; 최재운; 장이찬; Orlo H. Clark

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자료유형: 학술저널

저자정보: 유근수 이옥준 김원재 박성수 김동주 박진우 최재운 장이찬 Orlo H. Clark

저널정보: 대한갑상선-내분비외과학회 The Journal of Endocrine Surgery The Journal of Endocrine Surgery 제11권 제4호

발행연도: 2011.1

수록면: 234 - 241 (8page)

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Purpose: The Hedgehog (Hh) signaling pathway is important in embryonic development including cell differentiation and proliferation. Recently, activation of this pathway has been implicated in several forms of solid cancers. We investigated sonic hedgehog (Shh) protein expression and its relation to differentiation and clinicopathologic characteristics in thyroid cancer cell lines and tissues.Methods: We used five thyroid cancer cell lines: TPC-1, FTC-133, FTC-236, FTC-238, and XTC-1. We made tissue microarray slides using 80 thyroid surgical specimen: 40 benign and 40 malignant lesions. Immunohistochemical staining was performed using anti-Shh antibody. mRNA expression of NIS, thyroglobulin, and CD97 were evaluated by RT-PCR. Cyclopamine was used as a Shh signal inhibitor. Results: Shh expression was more prominent in TPC-1, FTC-133, and XTC-1 cell lines than the others. Cyclopamine downregulated CD97 and upregulated thyroglobulin mRNA expression, but did not induce mRNA expression of NIS. Thyroid tissues showed varied expression of Shh in both benign and malignant diseases. Shh expression was detected in 38 of 50 (76%) normal, in 18 of 25 (72%) non-neoplastic benign, in nine of 15 (60%) benign tumors, and in 31 of 40 (77%) malignant tumors. Shh over-expression was significantly less frequent in papillary thyroid carcinomas than in normal or benign thyroid tissues. In addition, Shh protein expression did not relate to clinicopathologic characteristics in papillary thyroid carcinomas. Conclusion: Thyroid tissues and cell lines vary in expression of Shh. Cyclopamine can induce redifferentiation in thyroid cancer cell lines. Shh protein expression, however, is unrelated to clinicopathologic characteristics in papillary thyroid carcinomas. (Korean J Endocrine Surg 2011;11:234-241)

#갑상선암 #재분화 #Sonic hedghog #Cyclopamine #Sonic hedghog #Cyclopamine #Thyroid cancer #Redifferentiation

참고문헌 (25)

참고문헌 신청

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