지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 27 - 33 (7page)
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Purpose: Vaginal epithelial cells have always been exposed to various pathogens. However, this has not always caused clinical infection. In addition to a previously reported protection effect of the vagina, currently, the innate immune response is thought to be important as one of the causes explaining the phenomenon. Therefore, we investigated the innate immunity of the vagina and related mechanisms in infected vaginal epithelial cells focusing on the antimicrobial peptide human β-defensin-3 (HBD-3).Materials and Methods: We investigated the signaling molecules, Toll-like receptors (TLRs), through which mammals sense infection in vaginal epithelial cells, with activation with lipopolysaccharide (LPS), Staphylococcus aureus peptidoglycan (PGN), or zymosan. Reverse transcriptase-polymerase chain reaction analysis of HBD-3 messenger RNA expression in vaginal epithelial cells after treatment with three pathogens was performed for investigation of pathogen-associated molecular patterns. Then, we also studied the following mechanism of innate immunity of the vagina focusing on HBD-3 in vaginal epithelial cells infected with gram-positive bacteria, gram-negative bacteria, or fungus.Results: Vaginal epithelial cells (VK2/E6E7 cells) constitutively expressed TLR2 and TLR4 and produced antimicrobial peptide HBD-3 upon activation with LPS, PGN, or zymosan. VK2/E6/E7 cells exposed to LPS, PGN, or zymosan showed increased p38 mitogen activated protein kinase (MAPK) activity. In addition, LPS-, PGN-, and zymosan-induced HBD-3 expression was attenuated by SB203580, a p38 MAPK inhibitor, emphasizing the importance of p38 MAPK in induction of HBD-3.Conclusions: Vaginal epithelial cells may contribute to the host innate immune defense upon exposure to gram-negative bacteria, gram-positive bacteria, or fungi in the vagina by upregulation of HBD-3 expression.
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참고문헌 (25)
참고문헌 신청
Akira S / 2001 / Toll-like receptors : critical proteins linking innate and acquired immunity / Nat Immunol 2 : 675 ~ 680
Albanesi C / 2007 / IL-4 and IL-13 negatively regulate TNF-alphaand IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling(SOCS)-1, and
Becker MN / 2000 / CD14-dependent lipopolysaccharide-induced beta-defensin-2 expression in human tracheobronchial epithelium / J Biol Chem 275 : 29731 ~ 29736
Ganz T / 1985 / Defensins. Natural peptide antibiotics of human neutrophils / J Clin Invest 76 : 1427 ~ 1435
Ganz T / 2004 / Antimicrobial polypeptides / J Leukoc Biol 75 : 34 ~ 38
Albanesi C / 2007 / IL-4 and IL-13 negatively regulate TNF-alphaand IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling(SOCS)-1, and
Becker MN / 2000 / CD14-dependent lipopolysaccharide-induced beta-defensin-2 expression in human tracheobronchial epithelium / J Biol Chem 275 : 29731 ~ 29736
Ganz T / 1985 / Defensins. Natural peptide antibiotics of human neutrophils / J Clin Invest 76 : 1427 ~ 1435
Ganz T / 2004 / Antimicrobial polypeptides / J Leukoc Biol 75 : 34 ~ 38
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