지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 99 - 103 (5page)
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초록· 키워드
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Purpose: In recent years, various diagnostic methods, including culture, immuno-logical detection, conventional polymerase chain reaction (PCR) based methods, and microarray experiment have been applied for detection of Mycoplasma genitalium and Ureaplasma urealyticum infection. We assayed results of real time PCR and culture of variable clinical samples and evaluated various diagnostic indexes for assessing the clinical usefulness of the Accupower UU Real-Time PCR Kit (Bioneer Corp.) for detection of U. urealyticum/parvum.Materials and Methods: We surveyed 111 results of culture test and antibiotic sensitivity test of Ureaplasma spp. that were requested to the department of laboratory medicine, National Police Hospital from January to April 2011. The specimens of Ureaplasma spp. were collected from 97 uterine cervical swab samples, 13 urine samples, and one expressed prostate secretion sample. Real-time PCR and culture methods were performed using the Accupower UU Real-Time PCR Kit (Bioneer Corp.) and Mycoplasma IST2 Kit (BioMérieux).Results: The real-time PCR results showed that 80 clinical specimens were infected with U. urealyticum/parvum. These results were compared with those confirmed by microbiological culture. Compared with the culture, the diagnostic indexes (sensitivity, specificity, positive predictive value, and negative predictive value) of Accupower UU Real-Time PCR were 88.6%, 38.8%, 48.8%, and 83.9%, and the concordance between the Accupower UU Real-Time PCR Kit and the microbio-logical culture method was 58.5%.Conclusions: Accupower UU Real-Time PCR is a very valuable technique which can process analysis of a massive number of samples with high speed, high sensitivity and specificity, and a high detection rate, particularly for Ureaplasma spp.
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참고문헌 (15)
참고문헌 신청
Abele-Horn M / 1996 / Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respir
Bae HG / 2003 / Detection of ureaplasma urealyticum and mycoplasma hominis in pregnant women using MYCOFAST(R)evolution 2 and PCR / Korean J Clin Microbiol 6 : 74 ~ 80
Biernat-Sudolska M / 2006 / Assessment of various diagnostic methods of ureaplasma respiratory tract infections in newborns / Acta Biochim Pol 53 : 609 ~ 611
Blanchard A / 1993 / Detection of Ureaplasma urealyticum by polymerase chain reaction in the urogenital tract of adults, in amniotic fluid, and in the respiratory tract of ne
Cao X / 2007 / Real-time TaqMan polymerase chain reaction assays for quantitative detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum / Diagn Micro
Bae HG / 2003 / Detection of ureaplasma urealyticum and mycoplasma hominis in pregnant women using MYCOFAST(R)evolution 2 and PCR / Korean J Clin Microbiol 6 : 74 ~ 80
Biernat-Sudolska M / 2006 / Assessment of various diagnostic methods of ureaplasma respiratory tract infections in newborns / Acta Biochim Pol 53 : 609 ~ 611
Blanchard A / 1993 / Detection of Ureaplasma urealyticum by polymerase chain reaction in the urogenital tract of adults, in amniotic fluid, and in the respiratory tract of ne
Cao X / 2007 / Real-time TaqMan polymerase chain reaction assays for quantitative detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum / Diagn Micro
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