지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 199 - 206 (8page)
이용수
초록· 키워드
오류제보하기
Purpose: Lung injury imposed by hyperoxia is the most important cause of bronchopulmonary dysplasia (BPD) in premature lungs, and hyperoxia has the chief biological effect of inducing cell death. Our objective was to investigate the response of cell death in fetal alveolar type II cells (FATIICs) exposed to different concentrations of hyperoxia for 36 h.
Methods: FATIICs were isolated on embryonic day 19 and exposed to 65%- or 85%-oxygen for 36 h. Cells in room air were used as controls. FACScan was performed in hyperoxic and control samples at 0/6/12/24/36 h, and the patterns of cell death were compared at each time point using flow-cytometry.
Results: Cell necrosis as measured by selective propidium iodide staining increased significantly from 12 h of 65%-hyperoxia and 6 h of 85%-hyperoxia, respectively. Cell necrosis increased 1.6-fold, 3.0-fold and 4.6-fold after 12 h, 24 h, and 36 h, respectively during 65%-hyperoxia and increased by 2.4-fold, 3.1-fold, 6.3-fold, and 8.8-fold after 6 h, 12 h, 24 h, and 36 h, respectively during 85%-hyperoxia compared to controls. Apoptotic cells as measured by selective Annexin-V staining peaked at 1.3% at 24 h of 65%-hyperoxia and peaked at 1.2% at 6 h of 85%-hyperoxia, respectively and then decreased rapidly.
Conclusion: This study shows that exposure to sublethal and lethal hyperoxia increases necrosis of FATIICs remarkably from the early stage of hyperoxia. These findings support the idea that short-term exposure to oxygen from birth may contribute to the evolution of “new” BPD in preterm lungs.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Barazzone C / 1998 / Oxygen toxicity in mouse lung: pathways to cell death / Am J Respir Cell Mol Biol 19 : 573 ~ 581
Barazzone C / 1999 / Keratinocyte growth factor protects alveolar epithelium and endothelium from oxygen-induced injury in mice / Am J Pathol 154 : 1479 ~ 1487
Barazzone C / 2000 / Mechanism of cell injury and death in hyperoxia: role of cytokines and Bcl-2 family proteins / Am J Respir Cell Mol Biol 22 : 517 ~ 519
Bhandari V / 2006 / Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death / Nat Med 12 : 1286 ~ 1293
Coalson JJ / 2003 / Pathology of new bronchopulmonary dysplasia / Semin Neonatol 8 : 73 ~ 81
Barazzone C / 1999 / Keratinocyte growth factor protects alveolar epithelium and endothelium from oxygen-induced injury in mice / Am J Pathol 154 : 1479 ~ 1487
Barazzone C / 2000 / Mechanism of cell injury and death in hyperoxia: role of cytokines and Bcl-2 family proteins / Am J Respir Cell Mol Biol 22 : 517 ~ 519
Bhandari V / 2006 / Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death / Nat Med 12 : 1286 ~ 1293
Coalson JJ / 2003 / Pathology of new bronchopulmonary dysplasia / Semin Neonatol 8 : 73 ~ 81
이 논문의 저자 정보
최근 본 자료
댓글(0)
0