지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Objectives : The purpose of the study is to investigate the protective effect of coenzyme Q10 on cytotoxicity effect of dental monomers in odontoblast(MDPC-23).
Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme Q10. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA.
Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme Q10 showed cell proliferation from 5 to 500 µM by WST-1 assay. Pre-treatment coenzyme Q10 showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme Q10 treatment group(group 2) in comparison with coenyme Q10 pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system.
Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme Q10 showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.
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참고문헌 (26)
참고문헌 신청
Bakopoulou A / 2009 / Molecular toxicology of substances released from resin-based dental restorative materials / Int J Mol Sci 10 (9) : 3961 ~ 3999
Brock GR / 2004 / Local and systemic total antioxidant capacity in periodontitis and health / J Clin Periodontol 31 (7) : 515 ~ 521
Chang HH / 2012 / Effect of triethylene glycol dimethacrylate on the cytotoxicity, cyclooxygenase-2 expression and prostanoids production in human dental pulp cells / Int En
Chen SJ / 2013 / Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches / Arch Gynecol Obstet 288 (1) : 19
Engel RH1 / 2006 / Oxidative stress and apoptosis: a new treatment paradigm in cancer / Front Biosci 11 : 300 ~ 312
Brock GR / 2004 / Local and systemic total antioxidant capacity in periodontitis and health / J Clin Periodontol 31 (7) : 515 ~ 521
Chang HH / 2012 / Effect of triethylene glycol dimethacrylate on the cytotoxicity, cyclooxygenase-2 expression and prostanoids production in human dental pulp cells / Int En
Chen SJ / 2013 / Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches / Arch Gynecol Obstet 288 (1) : 19
Engel RH1 / 2006 / Oxidative stress and apoptosis: a new treatment paradigm in cancer / Front Biosci 11 : 300 ~ 312
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