지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 56 - 63 (8page)
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초록· 키워드
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Objective: Ulmi Pumilae Cortex (UPC) is a deciduous tree with uneven pinnate leaves and is classified as a subfamily of Ulmuceae and contains many pharmacologically active constituents. The aim of this study was to investigate the effects of UPC on the growth and survival of AGS cells, the most common human gastric adenocarcinoma cell lines.
Methods: The AGS cells were treated with varying concentrations of UPC. Analyses of the sub G1,caspase-3 activity, and mitochondrial depolarization were conducted to determine whether AGS cell death occured by apoptosis.
Furthermore, to identify the role of the transient receptor potential melastatin (TRPM) 7 channels in AGS cell growth and survival, we used human embryonic kidney (HEK) 293 cells overexpressed with TRPM7 channels.
Results: The addition of UPC to a culture medium inhibited AGS cell growth and survival.
Experimental results showed that the sub G1,caspase-3 activity, and mitochondrial depolarization were increased. Furthermore,TRPM7 channel overexpression in HEK 293 cells exacerbated UPC-induced cell death.
Conclusion: These findings indicate that UPC inhibits the growth and survival of gastric cancer cells due to a blockade of the TRPM7 channel activity. Therefore, UPC is a potential drug for treatment of gastric cancer, and TRPM7 channels may play an important role in survival in cases of gastric cancer.
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참고문헌 (25)
참고문헌 신청
Abrams JM / 2002 / Competition and compensation: coupled to death in development and cancer / Cell 110 (4) : 403 ~ 406
Bharti AC / 2002 / Nuclear factor-kappa B and cancer: its role in prevention and therapy / Biochem Pharmacol 64 (5-6) : 883 ~ 888
Clapham DE / 2003 / TRP channels as cellular sensors / Nature 426 (6966) : 517 ~ 524
Cory S / 2002 / The Bcl2 family: regulators of the cellular life-or-death switch / Nat Rev Cancer 2 (9) : 647 ~ 656
Hellein KN / 2012 / A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR / J Microbiol Methods 89
Bharti AC / 2002 / Nuclear factor-kappa B and cancer: its role in prevention and therapy / Biochem Pharmacol 64 (5-6) : 883 ~ 888
Clapham DE / 2003 / TRP channels as cellular sensors / Nature 426 (6966) : 517 ~ 524
Cory S / 2002 / The Bcl2 family: regulators of the cellular life-or-death switch / Nat Rev Cancer 2 (9) : 647 ~ 656
Hellein KN / 2012 / A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR / J Microbiol Methods 89
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